Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability

Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages a...

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Detalles Bibliográficos
Autores principales: Harhala, Marek, Barylski, Jakub, Humińska-Lisowska, Kinga, Lecion, Dorota, Wojciechowicz, Jacek, Lahutta, Karolina, Kuś, Marta, Kropinski, Andrew M., Nowak, Sylwia, Nowicki, Grzegorz, Hodyra-Stefaniak, Katarzyna, Dąbrowska, Krystyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195288/
https://www.ncbi.nlm.nih.gov/pubmed/30339682
http://dx.doi.org/10.1371/journal.pone.0205995
Descripción
Sumario:Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages are members of the Siphoviridae, but their analysis revealed that Str01 and Str03 do not belong to any known genus. We identified their structural proteins based on LC–ESI29 MS/MS and list their basic thermal stability and physico-chemical features including optimum pH. Annotated genomic sequences of the phages are deposited in GenBank (NCBI accession numbers KY349816 and KY363359, respectively).

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