Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability

Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages a...

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Autores principales: Harhala, Marek, Barylski, Jakub, Humińska-Lisowska, Kinga, Lecion, Dorota, Wojciechowicz, Jacek, Lahutta, Karolina, Kuś, Marta, Kropinski, Andrew M., Nowak, Sylwia, Nowicki, Grzegorz, Hodyra-Stefaniak, Katarzyna, Dąbrowska, Krystyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195288/
https://www.ncbi.nlm.nih.gov/pubmed/30339682
http://dx.doi.org/10.1371/journal.pone.0205995
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author Harhala, Marek
Barylski, Jakub
Humińska-Lisowska, Kinga
Lecion, Dorota
Wojciechowicz, Jacek
Lahutta, Karolina
Kuś, Marta
Kropinski, Andrew M.
Nowak, Sylwia
Nowicki, Grzegorz
Hodyra-Stefaniak, Katarzyna
Dąbrowska, Krystyna
author_facet Harhala, Marek
Barylski, Jakub
Humińska-Lisowska, Kinga
Lecion, Dorota
Wojciechowicz, Jacek
Lahutta, Karolina
Kuś, Marta
Kropinski, Andrew M.
Nowak, Sylwia
Nowicki, Grzegorz
Hodyra-Stefaniak, Katarzyna
Dąbrowska, Krystyna
author_sort Harhala, Marek
collection PubMed
description Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages are members of the Siphoviridae, but their analysis revealed that Str01 and Str03 do not belong to any known genus. We identified their structural proteins based on LC–ESI29 MS/MS and list their basic thermal stability and physico-chemical features including optimum pH. Annotated genomic sequences of the phages are deposited in GenBank (NCBI accession numbers KY349816 and KY363359, respectively).
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spelling pubmed-61952882018-11-19 Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability Harhala, Marek Barylski, Jakub Humińska-Lisowska, Kinga Lecion, Dorota Wojciechowicz, Jacek Lahutta, Karolina Kuś, Marta Kropinski, Andrew M. Nowak, Sylwia Nowicki, Grzegorz Hodyra-Stefaniak, Katarzyna Dąbrowska, Krystyna PLoS One Research Article Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages are members of the Siphoviridae, but their analysis revealed that Str01 and Str03 do not belong to any known genus. We identified their structural proteins based on LC–ESI29 MS/MS and list their basic thermal stability and physico-chemical features including optimum pH. Annotated genomic sequences of the phages are deposited in GenBank (NCBI accession numbers KY349816 and KY363359, respectively). Public Library of Science 2018-10-19 /pmc/articles/PMC6195288/ /pubmed/30339682 http://dx.doi.org/10.1371/journal.pone.0205995 Text en © 2018 Harhala et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Harhala, Marek
Barylski, Jakub
Humińska-Lisowska, Kinga
Lecion, Dorota
Wojciechowicz, Jacek
Lahutta, Karolina
Kuś, Marta
Kropinski, Andrew M.
Nowak, Sylwia
Nowicki, Grzegorz
Hodyra-Stefaniak, Katarzyna
Dąbrowska, Krystyna
Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability
title Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability
title_full Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability
title_fullStr Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability
title_full_unstemmed Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability
title_short Two novel temperate bacteriophages infecting Streptococcus pyogenes: Their genomes, morphology and stability
title_sort two novel temperate bacteriophages infecting streptococcus pyogenes: their genomes, morphology and stability
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195288/
https://www.ncbi.nlm.nih.gov/pubmed/30339682
http://dx.doi.org/10.1371/journal.pone.0205995
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