Cargando…

Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation

The Acer truncatum Bunge is a particular widespread forest tree species in northern China. VLCFAs are important to eukaryotes survival and play diverse roles throughout the development. So far, there are reports that the Acer truncatum seeds fatty acid (FA) rich in VLCFAs, but little is known about...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Rongkai, Liu, Pei, Fan, Jinshuan, Li, Lingli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195533/
https://www.ncbi.nlm.nih.gov/pubmed/30341360
http://dx.doi.org/10.1038/s41598-018-33999-3
_version_ 1783364403978043392
author Wang, Rongkai
Liu, Pei
Fan, Jinshuan
Li, Lingli
author_facet Wang, Rongkai
Liu, Pei
Fan, Jinshuan
Li, Lingli
author_sort Wang, Rongkai
collection PubMed
description The Acer truncatum Bunge is a particular widespread forest tree species in northern China. VLCFAs are important to eukaryotes survival and play diverse roles throughout the development. So far, there are reports that the Acer truncatum seeds fatty acid (FA) rich in VLCFAs, but little is known about the physiological mechanism responsible for the biosynthesis. A total of approximately 37.07 Gbp was generated, it was comprehensive enough to determine the majority of the regulation VLCFAs biosynthesis genes. The 97,053 different unigenes were assembled and identified, and large numbers of EST-SSRs were determined. The expression profiles of crucial genes (KCS, KCR, HCD and ECR) involved in VLCFAs elongation of fatty acids were also studied. To our knowledge, the present study provides the first comprehensive of the transcriptome of Acer truncatum seeds. This transcriptome dataset have been made publicly available NCBI, we believe that it may provide new resource for future high-throughput gene expression of Acer truncatum seeds growth and development and will provide theoretical basic information for improving the yield of VLCFAs, especially nervonic acid.
format Online
Article
Text
id pubmed-6195533
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-61955332018-10-24 Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation Wang, Rongkai Liu, Pei Fan, Jinshuan Li, Lingli Sci Rep Article The Acer truncatum Bunge is a particular widespread forest tree species in northern China. VLCFAs are important to eukaryotes survival and play diverse roles throughout the development. So far, there are reports that the Acer truncatum seeds fatty acid (FA) rich in VLCFAs, but little is known about the physiological mechanism responsible for the biosynthesis. A total of approximately 37.07 Gbp was generated, it was comprehensive enough to determine the majority of the regulation VLCFAs biosynthesis genes. The 97,053 different unigenes were assembled and identified, and large numbers of EST-SSRs were determined. The expression profiles of crucial genes (KCS, KCR, HCD and ECR) involved in VLCFAs elongation of fatty acids were also studied. To our knowledge, the present study provides the first comprehensive of the transcriptome of Acer truncatum seeds. This transcriptome dataset have been made publicly available NCBI, we believe that it may provide new resource for future high-throughput gene expression of Acer truncatum seeds growth and development and will provide theoretical basic information for improving the yield of VLCFAs, especially nervonic acid. Nature Publishing Group UK 2018-10-19 /pmc/articles/PMC6195533/ /pubmed/30341360 http://dx.doi.org/10.1038/s41598-018-33999-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Rongkai
Liu, Pei
Fan, Jinshuan
Li, Lingli
Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation
title Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation
title_full Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation
title_fullStr Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation
title_full_unstemmed Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation
title_short Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation
title_sort comparative transcriptome analysis two genotypes of acer truncatum bunge seeds reveals candidate genes that influences seed vlcfas accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195533/
https://www.ncbi.nlm.nih.gov/pubmed/30341360
http://dx.doi.org/10.1038/s41598-018-33999-3
work_keys_str_mv AT wangrongkai comparativetranscriptomeanalysistwogenotypesofacertruncatumbungeseedsrevealscandidategenesthatinfluencesseedvlcfasaccumulation
AT liupei comparativetranscriptomeanalysistwogenotypesofacertruncatumbungeseedsrevealscandidategenesthatinfluencesseedvlcfasaccumulation
AT fanjinshuan comparativetranscriptomeanalysistwogenotypesofacertruncatumbungeseedsrevealscandidategenesthatinfluencesseedvlcfasaccumulation
AT lilingli comparativetranscriptomeanalysistwogenotypesofacertruncatumbungeseedsrevealscandidategenesthatinfluencesseedvlcfasaccumulation