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Small non-coding RNA landscape of extracellular vesicles from human stem cells

Extracellular vesicles (EVs) are reported to be involved in stem cell maintenance, self-renewal, and differentiation. Due to their bioactive cargoes influencing cell fate and function, interest in EVs in regenerative medicine has rapidly increased. EV-derived small non-coding RNA mimic the functions...

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Autores principales: Kaur, Sippy, Abu-Shahba, Ahmed G., Paananen, Riku O., Hongisto, Heidi, Hiidenmaa, Hanna, Skottman, Heli, Seppänen-Kaijansinkko, Riitta, Mannerström, Bettina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195565/
https://www.ncbi.nlm.nih.gov/pubmed/30341351
http://dx.doi.org/10.1038/s41598-018-33899-6
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author Kaur, Sippy
Abu-Shahba, Ahmed G.
Paananen, Riku O.
Hongisto, Heidi
Hiidenmaa, Hanna
Skottman, Heli
Seppänen-Kaijansinkko, Riitta
Mannerström, Bettina
author_facet Kaur, Sippy
Abu-Shahba, Ahmed G.
Paananen, Riku O.
Hongisto, Heidi
Hiidenmaa, Hanna
Skottman, Heli
Seppänen-Kaijansinkko, Riitta
Mannerström, Bettina
author_sort Kaur, Sippy
collection PubMed
description Extracellular vesicles (EVs) are reported to be involved in stem cell maintenance, self-renewal, and differentiation. Due to their bioactive cargoes influencing cell fate and function, interest in EVs in regenerative medicine has rapidly increased. EV-derived small non-coding RNA mimic the functions of the parent stem cells, regulating the maintenance and differentiation of stem cells, controlling the intercellular regulation of gene expression, and eventually affecting the cell fate. In this study, we used RNA sequencing to provide a comprehensive overview of the expression profiles of small non-coding transcripts carried by the EVs derived from human adipose tissue stromal/stem cells (AT-MSCs) and human pluripotent stem cells (hPSCs), both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSC). Both hPSCs and AT-MSCs were characterized and their EVs were extracted using standard protocols. Small non-coding RNA sequencing from EVs showed that hPSCs and AT-MSCs showed distinct profiles, unique for each stem cell source. Interestingly, in hPSCs, most abundant miRNAs were from specific miRNA families regulating pluripotency, reprogramming and differentiation (miR-17-92, mir-200, miR-302/367, miR-371/373, CM19 microRNA cluster). For the AT-MSCs, the highly expressed miRNAs were found to be regulating osteogenesis (let-7/98, miR-10/100, miR-125, miR-196, miR-199, miR-615-3p, mir-22-3p, mir-24-3p, mir-27a-3p, mir-193b-5p, mir-195-3p). Additionally, abundant small nuclear and nucleolar RNA were detected in hPSCs, whereas Y- and tRNA were found in AT-MSCs. Identification of EV-miRNA and non-coding RNA signatures released by these stem cells will provide clues towards understanding their role in intracellular communication, and well as their roles in maintaining the stem cell niche.
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spelling pubmed-61955652018-10-24 Small non-coding RNA landscape of extracellular vesicles from human stem cells Kaur, Sippy Abu-Shahba, Ahmed G. Paananen, Riku O. Hongisto, Heidi Hiidenmaa, Hanna Skottman, Heli Seppänen-Kaijansinkko, Riitta Mannerström, Bettina Sci Rep Article Extracellular vesicles (EVs) are reported to be involved in stem cell maintenance, self-renewal, and differentiation. Due to their bioactive cargoes influencing cell fate and function, interest in EVs in regenerative medicine has rapidly increased. EV-derived small non-coding RNA mimic the functions of the parent stem cells, regulating the maintenance and differentiation of stem cells, controlling the intercellular regulation of gene expression, and eventually affecting the cell fate. In this study, we used RNA sequencing to provide a comprehensive overview of the expression profiles of small non-coding transcripts carried by the EVs derived from human adipose tissue stromal/stem cells (AT-MSCs) and human pluripotent stem cells (hPSCs), both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSC). Both hPSCs and AT-MSCs were characterized and their EVs were extracted using standard protocols. Small non-coding RNA sequencing from EVs showed that hPSCs and AT-MSCs showed distinct profiles, unique for each stem cell source. Interestingly, in hPSCs, most abundant miRNAs were from specific miRNA families regulating pluripotency, reprogramming and differentiation (miR-17-92, mir-200, miR-302/367, miR-371/373, CM19 microRNA cluster). For the AT-MSCs, the highly expressed miRNAs were found to be regulating osteogenesis (let-7/98, miR-10/100, miR-125, miR-196, miR-199, miR-615-3p, mir-22-3p, mir-24-3p, mir-27a-3p, mir-193b-5p, mir-195-3p). Additionally, abundant small nuclear and nucleolar RNA were detected in hPSCs, whereas Y- and tRNA were found in AT-MSCs. Identification of EV-miRNA and non-coding RNA signatures released by these stem cells will provide clues towards understanding their role in intracellular communication, and well as their roles in maintaining the stem cell niche. Nature Publishing Group UK 2018-10-19 /pmc/articles/PMC6195565/ /pubmed/30341351 http://dx.doi.org/10.1038/s41598-018-33899-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kaur, Sippy
Abu-Shahba, Ahmed G.
Paananen, Riku O.
Hongisto, Heidi
Hiidenmaa, Hanna
Skottman, Heli
Seppänen-Kaijansinkko, Riitta
Mannerström, Bettina
Small non-coding RNA landscape of extracellular vesicles from human stem cells
title Small non-coding RNA landscape of extracellular vesicles from human stem cells
title_full Small non-coding RNA landscape of extracellular vesicles from human stem cells
title_fullStr Small non-coding RNA landscape of extracellular vesicles from human stem cells
title_full_unstemmed Small non-coding RNA landscape of extracellular vesicles from human stem cells
title_short Small non-coding RNA landscape of extracellular vesicles from human stem cells
title_sort small non-coding rna landscape of extracellular vesicles from human stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195565/
https://www.ncbi.nlm.nih.gov/pubmed/30341351
http://dx.doi.org/10.1038/s41598-018-33899-6
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