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Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids

Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeeded, via ra...

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Detalles Bibliográficos
Autores principales: Solovyev, Ilya D., Gavshina, Alexandra V., Katti, Aditya S., Chizhik, Alexey I., Vinokurov, Leonid M., Lapshin, Grigory D., Ivashina, Tatiana V., Khrenova, Maria G., Kireev, Igor I., Gregor, Ingo, Enderlein, Jörg, Savitsky, Alexander P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195611/
https://www.ncbi.nlm.nih.gov/pubmed/30341334
http://dx.doi.org/10.1038/s41598-018-33250-z