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CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis

CRISPR/Cas9 pooled screening permits parallel evaluation of comprehensive guide RNA libraries to systematically perturb protein coding sequences in situ and correlate with functional readouts. For the analysis and visualization of the resulting datasets, we develop CRISPRO, a computational pipeline...

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Detalles Bibliográficos
Autores principales:	Schoonenberg, Vivien A. C., Cole, Mitchel A., Yao, Qiuming, Macias-Treviño, Claudio, Sher, Falak, Schupp, Patrick G., Canver, Matthew C., Maeda, Takahiro, Pinello, Luca, Bauer, Daniel E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195731/ https://www.ncbi.nlm.nih.gov/pubmed/30340514 http://dx.doi.org/10.1186/s13059-018-1563-5

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