Cargando…

Robustness of biomarker determination in breast cancer by RT-qPCR: impact of tumor cell content, DCIS and non-neoplastic breast tissue

BACKGROUND: Tissue heterogeneity in formalin-fixed paraffin-embedded (FFPE) breast cancer specimens may affect the accuracy of reverse transcription quantitative real-time PCR (RT-qPCR). Herein, we tested the impact of tissue heterogeneity of breast cancer specimen on the RT-qPCR-based gene expressi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hartmann, Kerstin, Schlombs, Kornelia, Laible, Mark, Gürtler, Claudia, Schmidt, Marcus, Sahin, Ugur, Lehr, Hans-Anton
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195967/ https://www.ncbi.nlm.nih.gov/pubmed/30342538 http://dx.doi.org/10.1186/s13000-018-0760-6

Ejemplares similares

Biological subtyping of early breast cancer: a study comparing RT-qPCR with immunohistochemistry
por: Wirtz, Ralph M., et al.
Publicado: (2016)

Technical validation of an RT-qPCR in vitro diagnostic test system for the determination of breast cancer molecular subtypes by quantification of ERBB2, ESR1, PGR and MKI67 mRNA levels from formalin-fixed paraffin-embedded breast tumor specimens
por: Laible, Mark, et al.
Publicado: (2016)

Looking for more reliable biomarkers in breast cancer: Comparison between routine methods and RT-qPCR
por: Caselli, Emanuele, et al.
Publicado: (2021)

Predictive value of molecular subtyping in NMIBC by RT-qPCR of ERBB2, ESR1, PGR and MKI67 from formalin fixed TUR biopsies
por: Breyer, Johannes, et al.
Publicado: (2017)

SASqPCR: Robust and Rapid Analysis of RT-qPCR Data in SAS
por: Ling, Daijun
Publicado: (2012)

Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR
por: Strati, Areti, et al.
Publicado: (2011)

Concordance of RT-qPCR with immunohistochemistry and its beneficial role in breast cancer subtyping
por: Li, Yilun, et al.
Publicado: (2023)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

Detection of Circulating Tumour Cells from Blood of Breast Cancer Patients via RT-qPCR
por: Andergassen, Ulrich, et al.
Publicado: (2013)

PAM50 Breast Cancer Subtyping by RT-qPCR and Concordance with Standard Clinical Molecular Markers
por: Bastien, Roy RL, et al.
Publicado: (2012)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Genome-Wide Screening and Stability Verification of the Robust Internal Control Genes for RT-qPCR in Filamentous Fungi
por: Yang, Yayong, et al.
Publicado: (2022)

Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR
por: Stobnicka-Kupiec, Agata, et al.
Publicado: (2022)

Metasin—An Intra-Operative RT-qPCR Assay to Detect Metastatic Breast Cancer in Sentinel Lymph Nodes
por: Al-Ramadhani, Salma, et al.
Publicado: (2013)

High-throughput identification of reference genes for research and clinical RT-qPCR analysis of breast cancer samples
por: Maltseva, Diana V, et al.
Publicado: (2013)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

RT-qPCR Normalization Genes in the Red Alga Chondrus crispus
por: Kowalczyk, Nathalie, et al.
Publicado: (2014)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

Custom RT-qPCR-array for glaucoma filtering surgery prognosis
por: Rodriguez-Agirretxe, Iñaki, et al.
Publicado: (2017)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

Predictive factors of COVID-19 in patients with negative RT-qPCR
por: López de la Iglesia, J., et al.
Publicado: (2020)

Large-Scale RT-qPCR Diagnostics for Seed Potato Certification
por: Schumpp, Olivier, et al.
Publicado: (2021)

Sampling, Logistics, and Analytics of Urine for RT-qPCR-based Diagnostics
por: Kretschmer-Kazemi Far, Rosel, et al.
Publicado: (2021)

RT-qPCR-based pool testing for the diagnosis of COVID-19
por: Sato, Hugo Itaru, et al.
Publicado: (2023)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

NormQ: RNASeq normalization based on RT-qPCR derived size factors
por: Naraine, Ravindra, et al.
Publicado: (2020)

Identification of novel and robust internal control genes from Volvariella volvacea that are suitable for RT-qPCR in filamentous fungi
por: Tao, Yongxin, et al.
Publicado: (2016)

Performance of lateral flow assays for SARS-CoV-2 compared to RT-qPCR
por: Pilecky, Matthias, et al.
Publicado: (2022)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR
por: Shrestha, Kiran, et al.
Publicado: (2023)

Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA
por: Đermić, Damir, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_oium7a8mm49ivi1v9usl4fb65c