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MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats

Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. Currently, there i...

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Autores principales: Tuohy, James M., Mueller-Spitz, Sabrina R., Albert, Chad M., Scholz-Ng, Stacy E., Wall, Melinda E., Noutsios, George T., Gutierrez, Anthony J., Sandrin, Todd R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196315/
https://www.ncbi.nlm.nih.gov/pubmed/30374340
http://dx.doi.org/10.3389/fmicb.2018.02442
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author Tuohy, James M.
Mueller-Spitz, Sabrina R.
Albert, Chad M.
Scholz-Ng, Stacy E.
Wall, Melinda E.
Noutsios, George T.
Gutierrez, Anthony J.
Sandrin, Todd R.
author_facet Tuohy, James M.
Mueller-Spitz, Sabrina R.
Albert, Chad M.
Scholz-Ng, Stacy E.
Wall, Melinda E.
Noutsios, George T.
Gutierrez, Anthony J.
Sandrin, Todd R.
author_sort Tuohy, James M.
collection PubMed
description Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. Currently, there is keen interest in applying MALDI-TOF MS at taxonomic levels beyond species and to characterize environmental isolates. We constructed a model system consisting of 19 isolates of Deinococcus aquaticus obtained from biofilm communities indigenous to diverse substrates (concrete, leaf tissue, metal, and wood) in the Fox River – Lake Winnebago system of Wisconsin to: (1) develop rapid sample preparation methods that produce high quality, reproducible MALDI-TOF spectra and (2) compare the performance of MALDI-TOF MS-based profiling to common DNA-based approaches including 16S rRNA sequencing and genomic diversity by BOX-A1R fingerprinting. Our results suggest that MALDI-TOF MS can be used to rapidly and reproducibly characterize environmental isolates of D. aquaticus at the subpopulation level. MALDI-TOF MS provided higher taxonomic resolution than either 16S rRNA gene sequence analysis or BOX-A1R fingerprinting. Spectra contained features that appeared to permit characterization of isolates into two co-occurring subpopulations. However, reliable strain-level performance required rigorous and systematic standardization of culture conditions and sample preparation. Our work suggests that MALDI-TOF MS offers promise as a rapid, reproducible, and high-resolution approach to characterize environmental isolates of members of the genus Deinococcus. Future work will focus upon application of methods described here to additional members of this ecologically diverse and ubiquitous genus.
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spelling pubmed-61963152018-10-29 MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats Tuohy, James M. Mueller-Spitz, Sabrina R. Albert, Chad M. Scholz-Ng, Stacy E. Wall, Melinda E. Noutsios, George T. Gutierrez, Anthony J. Sandrin, Todd R. Front Microbiol Microbiology Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. Currently, there is keen interest in applying MALDI-TOF MS at taxonomic levels beyond species and to characterize environmental isolates. We constructed a model system consisting of 19 isolates of Deinococcus aquaticus obtained from biofilm communities indigenous to diverse substrates (concrete, leaf tissue, metal, and wood) in the Fox River – Lake Winnebago system of Wisconsin to: (1) develop rapid sample preparation methods that produce high quality, reproducible MALDI-TOF spectra and (2) compare the performance of MALDI-TOF MS-based profiling to common DNA-based approaches including 16S rRNA sequencing and genomic diversity by BOX-A1R fingerprinting. Our results suggest that MALDI-TOF MS can be used to rapidly and reproducibly characterize environmental isolates of D. aquaticus at the subpopulation level. MALDI-TOF MS provided higher taxonomic resolution than either 16S rRNA gene sequence analysis or BOX-A1R fingerprinting. Spectra contained features that appeared to permit characterization of isolates into two co-occurring subpopulations. However, reliable strain-level performance required rigorous and systematic standardization of culture conditions and sample preparation. Our work suggests that MALDI-TOF MS offers promise as a rapid, reproducible, and high-resolution approach to characterize environmental isolates of members of the genus Deinococcus. Future work will focus upon application of methods described here to additional members of this ecologically diverse and ubiquitous genus. Frontiers Media S.A. 2018-10-15 /pmc/articles/PMC6196315/ /pubmed/30374340 http://dx.doi.org/10.3389/fmicb.2018.02442 Text en Copyright © 2018 Tuohy, Mueller-Spitz, Albert, Scholz-Ng, Wall, Noutsios, Gutierrez and Sandrin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tuohy, James M.
Mueller-Spitz, Sabrina R.
Albert, Chad M.
Scholz-Ng, Stacy E.
Wall, Melinda E.
Noutsios, George T.
Gutierrez, Anthony J.
Sandrin, Todd R.
MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats
title MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats
title_full MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats
title_fullStr MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats
title_full_unstemmed MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats
title_short MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats
title_sort maldi-tof ms affords discrimination of deinococcus aquaticus isolates obtained from diverse biofilm habitats
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196315/
https://www.ncbi.nlm.nih.gov/pubmed/30374340
http://dx.doi.org/10.3389/fmicb.2018.02442
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