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Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili
Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies ha...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196383/ https://www.ncbi.nlm.nih.gov/pubmed/30296001 http://dx.doi.org/10.1111/1751-7915.13309 |
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author | Lienemann, Michael TerAvest, Michaela A. Pitkänen, Juha‐Pekka Stuns, Ingmar Penttilä, Merja Ajo‐Franklin, Caroline M. Jäntti, Jussi |
author_facet | Lienemann, Michael TerAvest, Michaela A. Pitkänen, Juha‐Pekka Stuns, Ingmar Penttilä, Merja Ajo‐Franklin, Caroline M. Jäntti, Jussi |
author_sort | Lienemann, Michael |
collection | PubMed |
description | Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR‐1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well‐characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose‐binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose‐coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals. |
format | Online Article Text |
id | pubmed-6196383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61963832018-10-30 Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili Lienemann, Michael TerAvest, Michaela A. Pitkänen, Juha‐Pekka Stuns, Ingmar Penttilä, Merja Ajo‐Franklin, Caroline M. Jäntti, Jussi Microb Biotechnol Research Articles Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR‐1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well‐characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose‐binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose‐coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals. John Wiley and Sons Inc. 2018-09-17 /pmc/articles/PMC6196383/ /pubmed/30296001 http://dx.doi.org/10.1111/1751-7915.13309 Text en © 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Lienemann, Michael TerAvest, Michaela A. Pitkänen, Juha‐Pekka Stuns, Ingmar Penttilä, Merja Ajo‐Franklin, Caroline M. Jäntti, Jussi Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili |
title | Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili |
title_full | Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili |
title_fullStr | Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili |
title_full_unstemmed | Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili |
title_short | Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili |
title_sort | towards patterned bioelectronics: facilitated immobilization of exoelectrogenic escherichia coli with heterologous pili |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196383/ https://www.ncbi.nlm.nih.gov/pubmed/30296001 http://dx.doi.org/10.1111/1751-7915.13309 |
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