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Random peptide mixtures as new crop protection agents

Many types of crops are severely affected by at least one important bacterial disease. Chemical control of bacterial plant diseases in the field vastly relies on copper‐based bactericides, yet with limited efficacy. In this study, we explored the potential of two random peptide mixture (RPM) models...

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Autores principales: Topman, Shiri, Tamir‐Ariel, Dafna, Bochnic‐Tamir, Heli, Stern Bauer, Tal, Shafir, Sharoni, Burdman, Saul, Hayouka, Zvi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196386/
https://www.ncbi.nlm.nih.gov/pubmed/29488347
http://dx.doi.org/10.1111/1751-7915.13258
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author Topman, Shiri
Tamir‐Ariel, Dafna
Bochnic‐Tamir, Heli
Stern Bauer, Tal
Shafir, Sharoni
Burdman, Saul
Hayouka, Zvi
author_facet Topman, Shiri
Tamir‐Ariel, Dafna
Bochnic‐Tamir, Heli
Stern Bauer, Tal
Shafir, Sharoni
Burdman, Saul
Hayouka, Zvi
author_sort Topman, Shiri
collection PubMed
description Many types of crops are severely affected by at least one important bacterial disease. Chemical control of bacterial plant diseases in the field vastly relies on copper‐based bactericides, yet with limited efficacy. In this study, we explored the potential of two random peptide mixture (RPM) models as novel crop protection agents. These unique peptide mixtures consist of random combination of l‐phenylalanine and l‐ or d‐lysine (FK‐20 and FdK‐20, respectively) along the 20 mer chain length of the peptides. Both RPMs displayed powerful bacteriostatic and bactericidal activities towards strains belonging to several plant pathogenic bacterial genera, for example, Xanthomonas, Clavibacter and Pseudomonas. In planta studies in the glasshouse revealed that RPMs significantly reduced disease severity of tomato and kohlrabi plants infected with Xanthomonas perforans and Xanthomonas campestris pv. campestris respectively. Moreover, RPM effects on reduction in disease severity were similar to those exerted by the commercial copper‐based bactericide Kocide 2000 that was applied at a 12‐fold higher concentration of the active compound relative to the RPM treatments. Importantly, the two tested RPM compounds had no toxic effect on survival of bees and Caco‐2 mammalian cells. This study demonstrates the potential of these innovative RPMs to serve as crop protection agents against crop diseases caused by phytopathogenic bacteria.
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spelling pubmed-61963862018-10-30 Random peptide mixtures as new crop protection agents Topman, Shiri Tamir‐Ariel, Dafna Bochnic‐Tamir, Heli Stern Bauer, Tal Shafir, Sharoni Burdman, Saul Hayouka, Zvi Microb Biotechnol Research Articles Many types of crops are severely affected by at least one important bacterial disease. Chemical control of bacterial plant diseases in the field vastly relies on copper‐based bactericides, yet with limited efficacy. In this study, we explored the potential of two random peptide mixture (RPM) models as novel crop protection agents. These unique peptide mixtures consist of random combination of l‐phenylalanine and l‐ or d‐lysine (FK‐20 and FdK‐20, respectively) along the 20 mer chain length of the peptides. Both RPMs displayed powerful bacteriostatic and bactericidal activities towards strains belonging to several plant pathogenic bacterial genera, for example, Xanthomonas, Clavibacter and Pseudomonas. In planta studies in the glasshouse revealed that RPMs significantly reduced disease severity of tomato and kohlrabi plants infected with Xanthomonas perforans and Xanthomonas campestris pv. campestris respectively. Moreover, RPM effects on reduction in disease severity were similar to those exerted by the commercial copper‐based bactericide Kocide 2000 that was applied at a 12‐fold higher concentration of the active compound relative to the RPM treatments. Importantly, the two tested RPM compounds had no toxic effect on survival of bees and Caco‐2 mammalian cells. This study demonstrates the potential of these innovative RPMs to serve as crop protection agents against crop diseases caused by phytopathogenic bacteria. John Wiley and Sons Inc. 2018-02-28 /pmc/articles/PMC6196386/ /pubmed/29488347 http://dx.doi.org/10.1111/1751-7915.13258 Text en © 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Topman, Shiri
Tamir‐Ariel, Dafna
Bochnic‐Tamir, Heli
Stern Bauer, Tal
Shafir, Sharoni
Burdman, Saul
Hayouka, Zvi
Random peptide mixtures as new crop protection agents
title Random peptide mixtures as new crop protection agents
title_full Random peptide mixtures as new crop protection agents
title_fullStr Random peptide mixtures as new crop protection agents
title_full_unstemmed Random peptide mixtures as new crop protection agents
title_short Random peptide mixtures as new crop protection agents
title_sort random peptide mixtures as new crop protection agents
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196386/
https://www.ncbi.nlm.nih.gov/pubmed/29488347
http://dx.doi.org/10.1111/1751-7915.13258
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