Production of a mono-biotinylated EGFR nanobody in the E. coli periplasm using the pET22b vector

OBJECTIVE: Our aim was to produce a mono-biotinylated single domain antibody (‘nanobody’) specific for the epidermal growth factor receptor (EGFR), which is overexpressed in many cancer cells. The binding of the nanobody and its function are tested in cancer cells. The construct could be used to car...

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Detalles Bibliográficos
Autores principales: Noor, Alfiah, Walser, Gudrun, Wesseling, Matthijs, Giron, Philippe, Laffra, Albert-Menno, Haddouchi, Fatima, De Grève, Jacques, Kronenberger, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196415/
https://www.ncbi.nlm.nih.gov/pubmed/30348204
http://dx.doi.org/10.1186/s13104-018-3852-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196415/
https://www.ncbi.nlm.nih.gov/pubmed/30348204
http://dx.doi.org/10.1186/s13104-018-3852-1

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