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Forensic application of comet assay: an emerging technique
Postmortem interval (PMI) estimation is a recurring problem in the field of forensic medicine. Conventional methods are effective but are insufficient to estimate accurate and precise time of death or PMI. In addition, degradation of biological samples is another major problem in forensic science wh...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197085/ https://www.ncbi.nlm.nih.gov/pubmed/30483639 http://dx.doi.org/10.1080/20961790.2017.1379893 |
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author | Shukla, Ritesh Kumar |
author_facet | Shukla, Ritesh Kumar |
author_sort | Shukla, Ritesh Kumar |
collection | PubMed |
description | Postmortem interval (PMI) estimation is a recurring problem in the field of forensic medicine. Conventional methods are effective but are insufficient to estimate accurate and precise time of death or PMI. In addition, degradation of biological samples is another major problem in forensic science which affects the investigation process and misleads the result. Some previous studies reported that DNA fragmentation has strong correlation with PMI. DNA fragmentation increased with prolonged PMI. Comet assay is a rapid sensitive, versatile, reliable and cost effective technique that is specifically used for qualitative and quantitative estimation of nuclear DNA fragmentation. Due to this attribute, comet assay can help to estimate accurate and precise time of death for some extent that is for early PMI estimation. In addition, two confounding factors are responsible for DNA fragmentation: (1) micro-organism; (2) environmental condition. Here, comet assay plays a dual role: (1) partially degraded samples get repaired using repair enzyme; (2) accurate time since deposition can be measured without using repair enzyme. Furthermore, this assay can also help to identify potential exposures of environmental-released chemicals/toxicants and its deleterious effects on human population. In this way, comet assay shows its versatile applications that could be useful for forensic investigation. Therefore, with the help of this review, an attempt was made to explore the versatility of comet assay technique for forensic applications and its future perspective. |
format | Online Article Text |
id | pubmed-6197085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-61970852018-11-27 Forensic application of comet assay: an emerging technique Shukla, Ritesh Kumar Forensic Sci Res Review Postmortem interval (PMI) estimation is a recurring problem in the field of forensic medicine. Conventional methods are effective but are insufficient to estimate accurate and precise time of death or PMI. In addition, degradation of biological samples is another major problem in forensic science which affects the investigation process and misleads the result. Some previous studies reported that DNA fragmentation has strong correlation with PMI. DNA fragmentation increased with prolonged PMI. Comet assay is a rapid sensitive, versatile, reliable and cost effective technique that is specifically used for qualitative and quantitative estimation of nuclear DNA fragmentation. Due to this attribute, comet assay can help to estimate accurate and precise time of death for some extent that is for early PMI estimation. In addition, two confounding factors are responsible for DNA fragmentation: (1) micro-organism; (2) environmental condition. Here, comet assay plays a dual role: (1) partially degraded samples get repaired using repair enzyme; (2) accurate time since deposition can be measured without using repair enzyme. Furthermore, this assay can also help to identify potential exposures of environmental-released chemicals/toxicants and its deleterious effects on human population. In this way, comet assay shows its versatile applications that could be useful for forensic investigation. Therefore, with the help of this review, an attempt was made to explore the versatility of comet assay technique for forensic applications and its future perspective. Taylor & Francis 2017-10-09 /pmc/articles/PMC6197085/ /pubmed/30483639 http://dx.doi.org/10.1080/20961790.2017.1379893 Text en © 2017 The Author(s). Published by Taylor & Francis Group on behalf of the Institute of Forensic Science, Ministry of Justice, People's Republic of China. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Shukla, Ritesh Kumar Forensic application of comet assay: an emerging technique |
title | Forensic application of comet assay: an emerging technique |
title_full | Forensic application of comet assay: an emerging technique |
title_fullStr | Forensic application of comet assay: an emerging technique |
title_full_unstemmed | Forensic application of comet assay: an emerging technique |
title_short | Forensic application of comet assay: an emerging technique |
title_sort | forensic application of comet assay: an emerging technique |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197085/ https://www.ncbi.nlm.nih.gov/pubmed/30483639 http://dx.doi.org/10.1080/20961790.2017.1379893 |
work_keys_str_mv | AT shuklariteshkumar forensicapplicationofcometassayanemergingtechnique |