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Quantitative analysis of opioids and cannabinoids in wastewater samples

Wastewater-based epidemiology is an innovative approach that uses the analysis of human excretion products in wastewater to obtain information about exposure to drugs in defined population groups. We developed and validated an analytical method for the simultaneous determination of opioids (morphine...

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Autores principales: Jacox, Alethea, Wetzel, Jillian, Cheng, Shu-Yuan, Concheiro, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197101/
https://www.ncbi.nlm.nih.gov/pubmed/30483615
http://dx.doi.org/10.1080/20961790.2016.1270812
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author Jacox, Alethea
Wetzel, Jillian
Cheng, Shu-Yuan
Concheiro, Marta
author_facet Jacox, Alethea
Wetzel, Jillian
Cheng, Shu-Yuan
Concheiro, Marta
author_sort Jacox, Alethea
collection PubMed
description Wastewater-based epidemiology is an innovative approach that uses the analysis of human excretion products in wastewater to obtain information about exposure to drugs in defined population groups. We developed and validated an analytical method for the simultaneous determination of opioids (morphine, oxycodone, hydrocodone, oxymorphone and hydromorphone), and cannabinoids (Δ(9)-tetrahydrocannabinol, 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) and THCCOOH-glucuronide) in raw-influent wastewater samples by ultra-high performance liquid chromatography-tandem mass spectrometry. Method validation included linearity (5–1 000 ng/L for opioids, 10–1 000 ng/L for cannabinoids), imprecision (<21.2%), accuracy (83%–131%), matrix effect (from –35.1% to –14.7%) and extraction efficiency (25%–84%), limit of detection (1–5 ng/L) and quantification (5–10 ng/L) and auto-sampler stability (no loss detected). River and wastewater samples were collected in triplicate from different locations in New York City and stored at −20 °C until analysis. Water from sewage overflow location tested positive for morphine (10.7 ng/L), oxycodone (4.2–23.5 ng/L), oxymorphone (4.8 ng/L) and hydromorphone (4.2 ng/L). Raw influent wastewater samples tested positive for morphine (133.0–258.3 ng/L), oxycodone (31.1–63.6 ng/L), oxymorphone (16.0–56.8 ng/L), hydromorphone (6.8–18.0 ng/L), hydrocodone (4.0–12.8 ng/L) and THCCOOH (168.2–772.0 ng/L). This method is sensitive and specific for opioids and marijuana determination in wastewater samples.
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spelling pubmed-61971012018-11-27 Quantitative analysis of opioids and cannabinoids in wastewater samples Jacox, Alethea Wetzel, Jillian Cheng, Shu-Yuan Concheiro, Marta Forensic Sci Res Original Article Wastewater-based epidemiology is an innovative approach that uses the analysis of human excretion products in wastewater to obtain information about exposure to drugs in defined population groups. We developed and validated an analytical method for the simultaneous determination of opioids (morphine, oxycodone, hydrocodone, oxymorphone and hydromorphone), and cannabinoids (Δ(9)-tetrahydrocannabinol, 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) and THCCOOH-glucuronide) in raw-influent wastewater samples by ultra-high performance liquid chromatography-tandem mass spectrometry. Method validation included linearity (5–1 000 ng/L for opioids, 10–1 000 ng/L for cannabinoids), imprecision (<21.2%), accuracy (83%–131%), matrix effect (from –35.1% to –14.7%) and extraction efficiency (25%–84%), limit of detection (1–5 ng/L) and quantification (5–10 ng/L) and auto-sampler stability (no loss detected). River and wastewater samples were collected in triplicate from different locations in New York City and stored at −20 °C until analysis. Water from sewage overflow location tested positive for morphine (10.7 ng/L), oxycodone (4.2–23.5 ng/L), oxymorphone (4.8 ng/L) and hydromorphone (4.2 ng/L). Raw influent wastewater samples tested positive for morphine (133.0–258.3 ng/L), oxycodone (31.1–63.6 ng/L), oxymorphone (16.0–56.8 ng/L), hydromorphone (6.8–18.0 ng/L), hydrocodone (4.0–12.8 ng/L) and THCCOOH (168.2–772.0 ng/L). This method is sensitive and specific for opioids and marijuana determination in wastewater samples. Taylor & Francis 2017-01-30 /pmc/articles/PMC6197101/ /pubmed/30483615 http://dx.doi.org/10.1080/20961790.2016.1270812 Text en © 2017 The Author(s). Published by Taylor & Francis Group on behalf of the Institute of Forensic Science, Ministry of Justice, People's Republic of China. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jacox, Alethea
Wetzel, Jillian
Cheng, Shu-Yuan
Concheiro, Marta
Quantitative analysis of opioids and cannabinoids in wastewater samples
title Quantitative analysis of opioids and cannabinoids in wastewater samples
title_full Quantitative analysis of opioids and cannabinoids in wastewater samples
title_fullStr Quantitative analysis of opioids and cannabinoids in wastewater samples
title_full_unstemmed Quantitative analysis of opioids and cannabinoids in wastewater samples
title_short Quantitative analysis of opioids and cannabinoids in wastewater samples
title_sort quantitative analysis of opioids and cannabinoids in wastewater samples
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197101/
https://www.ncbi.nlm.nih.gov/pubmed/30483615
http://dx.doi.org/10.1080/20961790.2016.1270812
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