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Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study

OBJECTIVE: The purpose of this study was to investigate the effects of the omentum, peritoneum, paratenon and skeletal muscle on the proliferation of the cartilage tissue using rabbit model as an in vivo culture medium. METHODS: 6 months old forty-five New Zealand rabbits were randomized into omentu...

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Autores principales: Buyukdogan, Kadir, Doral, Mahmut Nedim, Bilge, Onur, Turhan, Egemen, Huri, Gazi, Sargon, Mustafa Fevzi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Turkish Association of Orthopaedics and Traumatology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197382/
https://www.ncbi.nlm.nih.gov/pubmed/27717559
http://dx.doi.org/10.1016/j.aott.2016.08.003
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author Buyukdogan, Kadir
Doral, Mahmut Nedim
Bilge, Onur
Turhan, Egemen
Huri, Gazi
Sargon, Mustafa Fevzi
author_facet Buyukdogan, Kadir
Doral, Mahmut Nedim
Bilge, Onur
Turhan, Egemen
Huri, Gazi
Sargon, Mustafa Fevzi
author_sort Buyukdogan, Kadir
collection PubMed
description OBJECTIVE: The purpose of this study was to investigate the effects of the omentum, peritoneum, paratenon and skeletal muscle on the proliferation of the cartilage tissue using rabbit model as an in vivo culture medium. METHODS: 6 months old forty-five New Zealand rabbits were randomized into omentum, peritoneum, muscle, and Achilles paratenon groups. Standard sized osteochondral grafts were harvested from right knees and immediately placed into the specified tissues. Control group was fresh cartilage at the end of follow-up. After five months, samples were collected and evaluated macroscopically by measuring their dimensions (vertical = D1, horizontal = D2, and depth = D3) and volumes, and histologically by counting the chondrocyte number using camera lucida method. RESULTS: Macroscopically, increase in mean values for D1 and D2 dimensions of specimens from paratenon and omentum compared to pretransplant dimensions was statistically significant (p < 0.05). Although, volume measurements were higher in omentum and peritoneum group compared to pretransplant dimensions, increase was not significant (p > 0.05). Histologically, mean chondrocyte count was 14.0 ± 0.6 in fresh articular cartilage. Mean chondrocyte counts were 14.4 ± 0.9 in omentum group, 15.4 ± 1.0 in peritoneum group, 9.7 ± 1.3 in muscle group and 9.2 ± 0.4 in Achilles paratenon group respectively. However, mean chondrocyte counts were higher in samples of omentum and peritoneum group compared to fresh articular cartilage, increase was not statistically significant (p > 0.05). DISCUSSION: Transplantation of the cartilage grafts into mesothelium enhanced the chondrocyte counts and volumes compared with the pretransplant measurements. Mesothelium may have the potential to be used as an in vivo culture medium for osteochondral tissue growth.
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spelling pubmed-61973822018-10-24 Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study Buyukdogan, Kadir Doral, Mahmut Nedim Bilge, Onur Turhan, Egemen Huri, Gazi Sargon, Mustafa Fevzi Acta Orthop Traumatol Turc Original Article OBJECTIVE: The purpose of this study was to investigate the effects of the omentum, peritoneum, paratenon and skeletal muscle on the proliferation of the cartilage tissue using rabbit model as an in vivo culture medium. METHODS: 6 months old forty-five New Zealand rabbits were randomized into omentum, peritoneum, muscle, and Achilles paratenon groups. Standard sized osteochondral grafts were harvested from right knees and immediately placed into the specified tissues. Control group was fresh cartilage at the end of follow-up. After five months, samples were collected and evaluated macroscopically by measuring their dimensions (vertical = D1, horizontal = D2, and depth = D3) and volumes, and histologically by counting the chondrocyte number using camera lucida method. RESULTS: Macroscopically, increase in mean values for D1 and D2 dimensions of specimens from paratenon and omentum compared to pretransplant dimensions was statistically significant (p < 0.05). Although, volume measurements were higher in omentum and peritoneum group compared to pretransplant dimensions, increase was not significant (p > 0.05). Histologically, mean chondrocyte count was 14.0 ± 0.6 in fresh articular cartilage. Mean chondrocyte counts were 14.4 ± 0.9 in omentum group, 15.4 ± 1.0 in peritoneum group, 9.7 ± 1.3 in muscle group and 9.2 ± 0.4 in Achilles paratenon group respectively. However, mean chondrocyte counts were higher in samples of omentum and peritoneum group compared to fresh articular cartilage, increase was not statistically significant (p > 0.05). DISCUSSION: Transplantation of the cartilage grafts into mesothelium enhanced the chondrocyte counts and volumes compared with the pretransplant measurements. Mesothelium may have the potential to be used as an in vivo culture medium for osteochondral tissue growth. Turkish Association of Orthopaedics and Traumatology 2016-10 2016-10-05 /pmc/articles/PMC6197382/ /pubmed/27717559 http://dx.doi.org/10.1016/j.aott.2016.08.003 Text en © 2016 Turkish Association of Orthopaedics and Traumatology. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Buyukdogan, Kadir
Doral, Mahmut Nedim
Bilge, Onur
Turhan, Egemen
Huri, Gazi
Sargon, Mustafa Fevzi
Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study
title Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study
title_full Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study
title_fullStr Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study
title_full_unstemmed Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study
title_short Peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: A comparative animal study
title_sort peritoneum and omentum are natural reservoirs for chondrocytes of osteochondral autografts: a comparative animal study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197382/
https://www.ncbi.nlm.nih.gov/pubmed/27717559
http://dx.doi.org/10.1016/j.aott.2016.08.003
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