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Jejunal Metabolic Responses to Escherichia coli Infection in Piglets

This study aimed to investigate the jejunal metabolic variations in enterotoxigenic Escherichia coli (ETEC)-infected piglets. Piglets were infected with 1 × 10(10) CFUs (colony-forming units) of ETEC W25K and assigned into diarrheal, recovered, control, and resistant groups. Jejunal samples were har...

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Autores principales: Wu, Hucong, Liu, Jiaqi, Chen, Siyuan, Zhao, Yuanyuan, Zeng, Sijing, Bin, Peng, Zhang, Dong, Tang, Zhiyi, Zhu, Guoqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198047/
https://www.ncbi.nlm.nih.gov/pubmed/30386317
http://dx.doi.org/10.3389/fmicb.2018.02465
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author Wu, Hucong
Liu, Jiaqi
Chen, Siyuan
Zhao, Yuanyuan
Zeng, Sijing
Bin, Peng
Zhang, Dong
Tang, Zhiyi
Zhu, Guoqiang
author_facet Wu, Hucong
Liu, Jiaqi
Chen, Siyuan
Zhao, Yuanyuan
Zeng, Sijing
Bin, Peng
Zhang, Dong
Tang, Zhiyi
Zhu, Guoqiang
author_sort Wu, Hucong
collection PubMed
description This study aimed to investigate the jejunal metabolic variations in enterotoxigenic Escherichia coli (ETEC)-infected piglets. Piglets were infected with 1 × 10(10) CFUs (colony-forming units) of ETEC W25K and assigned into diarrheal, recovered, control, and resistant groups. Jejunal samples were harvested at day 6 and metabolic profiles were analyzed via gas chromatography coupled to time-of-flight mass spectrometry (GC/TOFMS). The results showed that 33 metabolites in the jejunum were identified in ETEC-induced diarrhea, including amino acids, fatty acids, sugars, and organic acids. Compared with the control, resistant, and recovered piglets, diarrheal piglets showed higher concentrations of 4-aminobutyric acid (GABA) and glycine in the jejunum. Compared with the control and resistant piglets, six metabolites were markedly decreased in diarrheal piglets, including ornithine, asparagine, glutamine, citric acid, citrulline, and lysine. Collectively, this study provides insights into jejunal metabolic response to ETEC infection and ETEC induced diarrhea in piglets.
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spelling pubmed-61980472018-11-01 Jejunal Metabolic Responses to Escherichia coli Infection in Piglets Wu, Hucong Liu, Jiaqi Chen, Siyuan Zhao, Yuanyuan Zeng, Sijing Bin, Peng Zhang, Dong Tang, Zhiyi Zhu, Guoqiang Front Microbiol Microbiology This study aimed to investigate the jejunal metabolic variations in enterotoxigenic Escherichia coli (ETEC)-infected piglets. Piglets were infected with 1 × 10(10) CFUs (colony-forming units) of ETEC W25K and assigned into diarrheal, recovered, control, and resistant groups. Jejunal samples were harvested at day 6 and metabolic profiles were analyzed via gas chromatography coupled to time-of-flight mass spectrometry (GC/TOFMS). The results showed that 33 metabolites in the jejunum were identified in ETEC-induced diarrhea, including amino acids, fatty acids, sugars, and organic acids. Compared with the control, resistant, and recovered piglets, diarrheal piglets showed higher concentrations of 4-aminobutyric acid (GABA) and glycine in the jejunum. Compared with the control and resistant piglets, six metabolites were markedly decreased in diarrheal piglets, including ornithine, asparagine, glutamine, citric acid, citrulline, and lysine. Collectively, this study provides insights into jejunal metabolic response to ETEC infection and ETEC induced diarrhea in piglets. Frontiers Media S.A. 2018-10-16 /pmc/articles/PMC6198047/ /pubmed/30386317 http://dx.doi.org/10.3389/fmicb.2018.02465 Text en Copyright © 2018 Wu, Liu, Chen, Zhao, Zeng, Bin, Zhang, Tang and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wu, Hucong
Liu, Jiaqi
Chen, Siyuan
Zhao, Yuanyuan
Zeng, Sijing
Bin, Peng
Zhang, Dong
Tang, Zhiyi
Zhu, Guoqiang
Jejunal Metabolic Responses to Escherichia coli Infection in Piglets
title Jejunal Metabolic Responses to Escherichia coli Infection in Piglets
title_full Jejunal Metabolic Responses to Escherichia coli Infection in Piglets
title_fullStr Jejunal Metabolic Responses to Escherichia coli Infection in Piglets
title_full_unstemmed Jejunal Metabolic Responses to Escherichia coli Infection in Piglets
title_short Jejunal Metabolic Responses to Escherichia coli Infection in Piglets
title_sort jejunal metabolic responses to escherichia coli infection in piglets
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198047/
https://www.ncbi.nlm.nih.gov/pubmed/30386317
http://dx.doi.org/10.3389/fmicb.2018.02465
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