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Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy

This article contains data related to the research article entitled “Novel approach for accurate minute DNA quantification on microvolumetric solutions” (Carvalho et al., 2018). The combination of PicoGreen® with a microvolume fluorospectrometer is a popular DNA quantification method due to its high...

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Detalles Bibliográficos
Autores principales: Carvalho, Joana, Negrinho, Renato, Azinheiro, Sarah, Garrido-Maestu, Alejandro, Barros-Velázquez, Jorge, Prado, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198060/
https://www.ncbi.nlm.nih.gov/pubmed/30364793
http://dx.doi.org/10.1016/j.dib.2018.09.098
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author Carvalho, Joana
Negrinho, Renato
Azinheiro, Sarah
Garrido-Maestu, Alejandro
Barros-Velázquez, Jorge
Prado, Marta
author_facet Carvalho, Joana
Negrinho, Renato
Azinheiro, Sarah
Garrido-Maestu, Alejandro
Barros-Velázquez, Jorge
Prado, Marta
author_sort Carvalho, Joana
collection PubMed
description This article contains data related to the research article entitled “Novel approach for accurate minute DNA quantification on microvolumetric solutions” (Carvalho et al., 2018). The combination of PicoGreen® with a microvolume fluorospectrometer is a popular DNA quantification method due to its high sensitivity and minimal consumption of sample, being commonly used to evaluate the performance of microfluidic devices designed for DNA purification. In this study, the authors present data related with the effect of DNA fragmentation level. The present data article includes the data used on the precision evaluation, in terms of repeatability, of the mathematical models developed to obtain the standards curve for salmon sperm DNA (low molecular weight). In addition, results related with the effect of some compounds on the DNA quantification accuracy using λDNA are presented.
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spelling pubmed-61980602018-10-25 Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy Carvalho, Joana Negrinho, Renato Azinheiro, Sarah Garrido-Maestu, Alejandro Barros-Velázquez, Jorge Prado, Marta Data Brief Chemistry This article contains data related to the research article entitled “Novel approach for accurate minute DNA quantification on microvolumetric solutions” (Carvalho et al., 2018). The combination of PicoGreen® with a microvolume fluorospectrometer is a popular DNA quantification method due to its high sensitivity and minimal consumption of sample, being commonly used to evaluate the performance of microfluidic devices designed for DNA purification. In this study, the authors present data related with the effect of DNA fragmentation level. The present data article includes the data used on the precision evaluation, in terms of repeatability, of the mathematical models developed to obtain the standards curve for salmon sperm DNA (low molecular weight). In addition, results related with the effect of some compounds on the DNA quantification accuracy using λDNA are presented. Elsevier 2018-10-02 /pmc/articles/PMC6198060/ /pubmed/30364793 http://dx.doi.org/10.1016/j.dib.2018.09.098 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Chemistry
Carvalho, Joana
Negrinho, Renato
Azinheiro, Sarah
Garrido-Maestu, Alejandro
Barros-Velázquez, Jorge
Prado, Marta
Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy
title Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy
title_full Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy
title_fullStr Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy
title_full_unstemmed Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy
title_short Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy
title_sort data on minute dna quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the dna quantification accuracy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198060/
https://www.ncbi.nlm.nih.gov/pubmed/30364793
http://dx.doi.org/10.1016/j.dib.2018.09.098
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