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Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy
This article contains data related to the research article entitled “Novel approach for accurate minute DNA quantification on microvolumetric solutions” (Carvalho et al., 2018). The combination of PicoGreen® with a microvolume fluorospectrometer is a popular DNA quantification method due to its high...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198060/ https://www.ncbi.nlm.nih.gov/pubmed/30364793 http://dx.doi.org/10.1016/j.dib.2018.09.098 |
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author | Carvalho, Joana Negrinho, Renato Azinheiro, Sarah Garrido-Maestu, Alejandro Barros-Velázquez, Jorge Prado, Marta |
author_facet | Carvalho, Joana Negrinho, Renato Azinheiro, Sarah Garrido-Maestu, Alejandro Barros-Velázquez, Jorge Prado, Marta |
author_sort | Carvalho, Joana |
collection | PubMed |
description | This article contains data related to the research article entitled “Novel approach for accurate minute DNA quantification on microvolumetric solutions” (Carvalho et al., 2018). The combination of PicoGreen® with a microvolume fluorospectrometer is a popular DNA quantification method due to its high sensitivity and minimal consumption of sample, being commonly used to evaluate the performance of microfluidic devices designed for DNA purification. In this study, the authors present data related with the effect of DNA fragmentation level. The present data article includes the data used on the precision evaluation, in terms of repeatability, of the mathematical models developed to obtain the standards curve for salmon sperm DNA (low molecular weight). In addition, results related with the effect of some compounds on the DNA quantification accuracy using λDNA are presented. |
format | Online Article Text |
id | pubmed-6198060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61980602018-10-25 Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy Carvalho, Joana Negrinho, Renato Azinheiro, Sarah Garrido-Maestu, Alejandro Barros-Velázquez, Jorge Prado, Marta Data Brief Chemistry This article contains data related to the research article entitled “Novel approach for accurate minute DNA quantification on microvolumetric solutions” (Carvalho et al., 2018). The combination of PicoGreen® with a microvolume fluorospectrometer is a popular DNA quantification method due to its high sensitivity and minimal consumption of sample, being commonly used to evaluate the performance of microfluidic devices designed for DNA purification. In this study, the authors present data related with the effect of DNA fragmentation level. The present data article includes the data used on the precision evaluation, in terms of repeatability, of the mathematical models developed to obtain the standards curve for salmon sperm DNA (low molecular weight). In addition, results related with the effect of some compounds on the DNA quantification accuracy using λDNA are presented. Elsevier 2018-10-02 /pmc/articles/PMC6198060/ /pubmed/30364793 http://dx.doi.org/10.1016/j.dib.2018.09.098 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Chemistry Carvalho, Joana Negrinho, Renato Azinheiro, Sarah Garrido-Maestu, Alejandro Barros-Velázquez, Jorge Prado, Marta Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy |
title | Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy |
title_full | Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy |
title_fullStr | Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy |
title_full_unstemmed | Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy |
title_short | Data on minute DNA quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the DNA quantification accuracy |
title_sort | data on minute dna quantification on microvolumetric solutions: comparison of mathematical models and effect of some compounds on the dna quantification accuracy |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198060/ https://www.ncbi.nlm.nih.gov/pubmed/30364793 http://dx.doi.org/10.1016/j.dib.2018.09.098 |
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