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High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment

OBJECTIVE: We tested the effect of exercise training and genistein treatment on splenomegaly in mice fed a high-fat, high-sugar diet (HFSD). RESULTS: Male and female C57BL6 mice fed HFSD containing 60% fat along with drinking water containing 42 g/L sugar (55% sucrose/45% fructose) for 12 weeks exhi...

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Autores principales: Buchan, Levi, St. Aubin, Chaheyla R., Fisher, Amy L., Hellings, Austin, Castro, Monica, Al-Nakkash, Layla, Broderick, Tom L., Plochocki, Jeffrey H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198361/
https://www.ncbi.nlm.nih.gov/pubmed/30348225
http://dx.doi.org/10.1186/s13104-018-3862-z
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author Buchan, Levi
St. Aubin, Chaheyla R.
Fisher, Amy L.
Hellings, Austin
Castro, Monica
Al-Nakkash, Layla
Broderick, Tom L.
Plochocki, Jeffrey H.
author_facet Buchan, Levi
St. Aubin, Chaheyla R.
Fisher, Amy L.
Hellings, Austin
Castro, Monica
Al-Nakkash, Layla
Broderick, Tom L.
Plochocki, Jeffrey H.
author_sort Buchan, Levi
collection PubMed
description OBJECTIVE: We tested the effect of exercise training and genistein treatment on splenomegaly in mice fed a high-fat, high-sugar diet (HFSD). RESULTS: Male and female C57BL6 mice fed HFSD containing 60% fat along with drinking water containing 42 g/L sugar (55% sucrose/45% fructose) for 12 weeks exhibited significant obesity, hyperglycemia, and elevated plasma IL-6 levels. This was accompanied by splenomegaly characterized by spleen weights 50% larger than mice fed standard chow (P < 0.05) with enlarged rad and white pulps. Mice fed HFSD and treated with a combination of exercise (30 min/day, 5 days/week) and genistein (600 mg genistein/kg diet) had reduced spleen weight (P < 0.05). The decrease in spleen weight was associated with a significant improvement in red-to-white pulp area ratio and plasma glucose and IL-6 (P < 0.05). Our findings indicate that reversal of splenomegaly by regular exercise and genistein treatment may be important in the clinical management of HFSD-induced obesity.
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spelling pubmed-61983612018-10-30 High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment Buchan, Levi St. Aubin, Chaheyla R. Fisher, Amy L. Hellings, Austin Castro, Monica Al-Nakkash, Layla Broderick, Tom L. Plochocki, Jeffrey H. BMC Res Notes Research Note OBJECTIVE: We tested the effect of exercise training and genistein treatment on splenomegaly in mice fed a high-fat, high-sugar diet (HFSD). RESULTS: Male and female C57BL6 mice fed HFSD containing 60% fat along with drinking water containing 42 g/L sugar (55% sucrose/45% fructose) for 12 weeks exhibited significant obesity, hyperglycemia, and elevated plasma IL-6 levels. This was accompanied by splenomegaly characterized by spleen weights 50% larger than mice fed standard chow (P < 0.05) with enlarged rad and white pulps. Mice fed HFSD and treated with a combination of exercise (30 min/day, 5 days/week) and genistein (600 mg genistein/kg diet) had reduced spleen weight (P < 0.05). The decrease in spleen weight was associated with a significant improvement in red-to-white pulp area ratio and plasma glucose and IL-6 (P < 0.05). Our findings indicate that reversal of splenomegaly by regular exercise and genistein treatment may be important in the clinical management of HFSD-induced obesity. BioMed Central 2018-10-22 /pmc/articles/PMC6198361/ /pubmed/30348225 http://dx.doi.org/10.1186/s13104-018-3862-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Buchan, Levi
St. Aubin, Chaheyla R.
Fisher, Amy L.
Hellings, Austin
Castro, Monica
Al-Nakkash, Layla
Broderick, Tom L.
Plochocki, Jeffrey H.
High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment
title High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment
title_full High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment
title_fullStr High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment
title_full_unstemmed High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment
title_short High-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment
title_sort high-fat, high-sugar diet induces splenomegaly that is ameliorated with exercise and genistein treatment
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198361/
https://www.ncbi.nlm.nih.gov/pubmed/30348225
http://dx.doi.org/10.1186/s13104-018-3862-z
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