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Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach
Skeletal muscle is an archetypal organ whose structure is tuned to match function. The magnitude of order in muscle fibers and myofibrils containing motor protein polymers determines the directed force output of the summed force vectors and, therefore, the muscle’s power performance on the structura...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199289/ https://www.ncbi.nlm.nih.gov/pubmed/30374401 http://dx.doi.org/10.1038/s41377-018-0080-3 |
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author | Schneidereit, Dominik Nübler, Stefanie Prölß, Gerhard Reischl, Barbara Schürmann, Sebastian Müller, Oliver J Friedrich, Oliver |
author_facet | Schneidereit, Dominik Nübler, Stefanie Prölß, Gerhard Reischl, Barbara Schürmann, Sebastian Müller, Oliver J Friedrich, Oliver |
author_sort | Schneidereit, Dominik |
collection | PubMed |
description | Skeletal muscle is an archetypal organ whose structure is tuned to match function. The magnitude of order in muscle fibers and myofibrils containing motor protein polymers determines the directed force output of the summed force vectors and, therefore, the muscle’s power performance on the structural level. Structure and function can change dramatically during disease states involving chronic remodeling. Cellular remodeling of the cytoarchitecture has been pursued using noninvasive and label-free multiphoton second harmonic generation (SHG) microscopy. Hereby, structure parameters can be extracted as a measure of myofibrillar order and thus are suggestive of the force output that a remodeled structure can still achieve. However, to date, the parameters have only been an indirect measure, and a precise calibration of optical SHG assessment for an exerted force has been elusive as no technology in existence correlates these factors. We engineered a novel, automated, high-precision biomechatronics system into a multiphoton microscope allows simultaneous isometric Ca(2+)-graded force or passive viscoelasticity measurements and SHG recordings. Using this MechaMorph system, we studied force and SHG in single EDL muscle fibers from wt and mdx mice; the latter serves as a model for compromised force and abnormal myofibrillar structure. We present Ca(2+)-graded isometric force, pCa-force curves, passive viscoelastic parameters and 3D structure in the same fiber for the first time. Furthermore, we provide a direct calibration of isometric force to morphology, which allows noninvasive prediction of the force output of single fibers from only multiphoton images, suggesting a potential application in the diagnosis of myopathies. |
format | Online Article Text |
id | pubmed-6199289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61992892018-10-29 Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach Schneidereit, Dominik Nübler, Stefanie Prölß, Gerhard Reischl, Barbara Schürmann, Sebastian Müller, Oliver J Friedrich, Oliver Light Sci Appl Article Skeletal muscle is an archetypal organ whose structure is tuned to match function. The magnitude of order in muscle fibers and myofibrils containing motor protein polymers determines the directed force output of the summed force vectors and, therefore, the muscle’s power performance on the structural level. Structure and function can change dramatically during disease states involving chronic remodeling. Cellular remodeling of the cytoarchitecture has been pursued using noninvasive and label-free multiphoton second harmonic generation (SHG) microscopy. Hereby, structure parameters can be extracted as a measure of myofibrillar order and thus are suggestive of the force output that a remodeled structure can still achieve. However, to date, the parameters have only been an indirect measure, and a precise calibration of optical SHG assessment for an exerted force has been elusive as no technology in existence correlates these factors. We engineered a novel, automated, high-precision biomechatronics system into a multiphoton microscope allows simultaneous isometric Ca(2+)-graded force or passive viscoelasticity measurements and SHG recordings. Using this MechaMorph system, we studied force and SHG in single EDL muscle fibers from wt and mdx mice; the latter serves as a model for compromised force and abnormal myofibrillar structure. We present Ca(2+)-graded isometric force, pCa-force curves, passive viscoelastic parameters and 3D structure in the same fiber for the first time. Furthermore, we provide a direct calibration of isometric force to morphology, which allows noninvasive prediction of the force output of single fibers from only multiphoton images, suggesting a potential application in the diagnosis of myopathies. Nature Publishing Group UK 2018-10-24 /pmc/articles/PMC6199289/ /pubmed/30374401 http://dx.doi.org/10.1038/s41377-018-0080-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Schneidereit, Dominik Nübler, Stefanie Prölß, Gerhard Reischl, Barbara Schürmann, Sebastian Müller, Oliver J Friedrich, Oliver Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach |
title | Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach |
title_full | Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach |
title_fullStr | Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach |
title_full_unstemmed | Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach |
title_short | Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach |
title_sort | optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199289/ https://www.ncbi.nlm.nih.gov/pubmed/30374401 http://dx.doi.org/10.1038/s41377-018-0080-3 |
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