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Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection
Lack of biomarkers for treatment monitoring is listed among the main requirements for next generation assays, as identified globally among tuberculosis (TB) researchers. In this study, we evaluated in a low TB endemic country such as Italy, the effect of preventive therapy on the results obtained in...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199315/ https://www.ncbi.nlm.nih.gov/pubmed/30353115 http://dx.doi.org/10.1038/s41598-018-33825-w |
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author | Petruccioli, Elisa Chiacchio, Teresa Vanini, Valentina Cuzzi, Gilda Codecasa, Luigi Ruffo Ferrarese, Maurizio Schininà, Vincenzo Palmieri, Fabrizio Ippolito, Giuseppe Goletti, Delia |
author_facet | Petruccioli, Elisa Chiacchio, Teresa Vanini, Valentina Cuzzi, Gilda Codecasa, Luigi Ruffo Ferrarese, Maurizio Schininà, Vincenzo Palmieri, Fabrizio Ippolito, Giuseppe Goletti, Delia |
author_sort | Petruccioli, Elisa |
collection | PubMed |
description | Lack of biomarkers for treatment monitoring is listed among the main requirements for next generation assays, as identified globally among tuberculosis (TB) researchers. In this study, we evaluated in a low TB endemic country such as Italy, the effect of preventive therapy on the results obtained in the QuantiFERON TB Plus (QFT-Plus), in a cohort of subjects with latent TB infection (LTBI) and active TB. We found that TB therapy significantly decreased IFN-γ values and number of responders to TB1- and TB2- peptides stimulation in both LTBI and active TB patients. Stratifying LTBI subjects according to the type of preventive TB therapy used, we found that INH treatment but not INH and RIF significantly decreased IFN-γ production. Stratifying the active TB patients according the microbiological status, we found that TB therapy significantly decreased IFN-γ response to antigen present in QFT-Plus test in patients with clinical diagnosis compared to those with a microbiological diagnosis. In conclusions, we demonstrated that TB therapy decreases IFN-γ level in response to antigen present in QFT-Plus test in LTBI and active TB patients. Future studies are needed to better characterize Mtb-specifc response as a potential marker for monitoring TB therapy and preventive treatment effects. |
format | Online Article Text |
id | pubmed-6199315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61993152018-10-25 Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection Petruccioli, Elisa Chiacchio, Teresa Vanini, Valentina Cuzzi, Gilda Codecasa, Luigi Ruffo Ferrarese, Maurizio Schininà, Vincenzo Palmieri, Fabrizio Ippolito, Giuseppe Goletti, Delia Sci Rep Article Lack of biomarkers for treatment monitoring is listed among the main requirements for next generation assays, as identified globally among tuberculosis (TB) researchers. In this study, we evaluated in a low TB endemic country such as Italy, the effect of preventive therapy on the results obtained in the QuantiFERON TB Plus (QFT-Plus), in a cohort of subjects with latent TB infection (LTBI) and active TB. We found that TB therapy significantly decreased IFN-γ values and number of responders to TB1- and TB2- peptides stimulation in both LTBI and active TB patients. Stratifying LTBI subjects according to the type of preventive TB therapy used, we found that INH treatment but not INH and RIF significantly decreased IFN-γ production. Stratifying the active TB patients according the microbiological status, we found that TB therapy significantly decreased IFN-γ response to antigen present in QFT-Plus test in patients with clinical diagnosis compared to those with a microbiological diagnosis. In conclusions, we demonstrated that TB therapy decreases IFN-γ level in response to antigen present in QFT-Plus test in LTBI and active TB patients. Future studies are needed to better characterize Mtb-specifc response as a potential marker for monitoring TB therapy and preventive treatment effects. Nature Publishing Group UK 2018-10-23 /pmc/articles/PMC6199315/ /pubmed/30353115 http://dx.doi.org/10.1038/s41598-018-33825-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Petruccioli, Elisa Chiacchio, Teresa Vanini, Valentina Cuzzi, Gilda Codecasa, Luigi Ruffo Ferrarese, Maurizio Schininà, Vincenzo Palmieri, Fabrizio Ippolito, Giuseppe Goletti, Delia Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection |
title | Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection |
title_full | Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection |
title_fullStr | Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection |
title_full_unstemmed | Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection |
title_short | Effect of therapy on Quantiferon-Plus response in patients with active and latent tuberculosis infection |
title_sort | effect of therapy on quantiferon-plus response in patients with active and latent tuberculosis infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199315/ https://www.ncbi.nlm.nih.gov/pubmed/30353115 http://dx.doi.org/10.1038/s41598-018-33825-w |
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