Cargando…

Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia

BACKGROUND: The treatment of congenital pseudoarthrosis of the tibia (CPT) remains challenging in pediatric orthopedics due to the difficulties in bone union, continuous angulation, joint stiffness, and severe limb length discrepancy. Mesenchymal stem cells (MSCs) therapy offers a complementary appr...

Descripción completa

Detalles Bibliográficos
Autores principales: Dilogo, Ismail Hadisoebroto, Mujadid, Fajar, Nurhayati, Retno Wahyu, Kurniawan, Aryadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199809/
https://www.ncbi.nlm.nih.gov/pubmed/30352605
http://dx.doi.org/10.1186/s13018-018-0977-9
_version_ 1783365206477373440
author Dilogo, Ismail Hadisoebroto
Mujadid, Fajar
Nurhayati, Retno Wahyu
Kurniawan, Aryadi
author_facet Dilogo, Ismail Hadisoebroto
Mujadid, Fajar
Nurhayati, Retno Wahyu
Kurniawan, Aryadi
author_sort Dilogo, Ismail Hadisoebroto
collection PubMed
description BACKGROUND: The treatment of congenital pseudoarthrosis of the tibia (CPT) remains challenging in pediatric orthopedics due to the difficulties in bone union, continuous angulation, joint stiffness, and severe limb length discrepancy. Mesenchymal stem cells (MSCs) therapy offers a complementary approach to improve the conventional surgical treatments. Although the autologous MSC treatment shows a promising strategy to promote bone healing in CPT patients, the quality of MSCs from CPT patients has not been well studied. The purpose of this study is to investigate the quality of MSCs isolated from patients with CPT. METHODS: The bone marrow-derived MSCs from the fracture site and iliac crest of six CPT patients were isolated and compared. The cumulative population doubling level (cPDL), phenotype characteristics, and trilineage differentiation potency were observed to assess the quality of both MSCs. RESULTS: There were no significant differences of the MSCs derived from the fracture site and the MSCs from the iliac crest of the subjects, in terms of cPDL, phenotype characteristics, and trilineage differentiation potency (all p > 0.05). However, MSCs from the fracture site had a higher senescence tendency than those from the iliac crest. CONCLUSION: MSC quality is not the main reason for delayed bone regeneration in those with CPT. Thus, autologous MSC is a promising source for treating CPT patients
format Online
Article
Text
id pubmed-6199809
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-61998092018-10-31 Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia Dilogo, Ismail Hadisoebroto Mujadid, Fajar Nurhayati, Retno Wahyu Kurniawan, Aryadi J Orthop Surg Res Research Article BACKGROUND: The treatment of congenital pseudoarthrosis of the tibia (CPT) remains challenging in pediatric orthopedics due to the difficulties in bone union, continuous angulation, joint stiffness, and severe limb length discrepancy. Mesenchymal stem cells (MSCs) therapy offers a complementary approach to improve the conventional surgical treatments. Although the autologous MSC treatment shows a promising strategy to promote bone healing in CPT patients, the quality of MSCs from CPT patients has not been well studied. The purpose of this study is to investigate the quality of MSCs isolated from patients with CPT. METHODS: The bone marrow-derived MSCs from the fracture site and iliac crest of six CPT patients were isolated and compared. The cumulative population doubling level (cPDL), phenotype characteristics, and trilineage differentiation potency were observed to assess the quality of both MSCs. RESULTS: There were no significant differences of the MSCs derived from the fracture site and the MSCs from the iliac crest of the subjects, in terms of cPDL, phenotype characteristics, and trilineage differentiation potency (all p > 0.05). However, MSCs from the fracture site had a higher senescence tendency than those from the iliac crest. CONCLUSION: MSC quality is not the main reason for delayed bone regeneration in those with CPT. Thus, autologous MSC is a promising source for treating CPT patients BioMed Central 2018-10-23 /pmc/articles/PMC6199809/ /pubmed/30352605 http://dx.doi.org/10.1186/s13018-018-0977-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Dilogo, Ismail Hadisoebroto
Mujadid, Fajar
Nurhayati, Retno Wahyu
Kurniawan, Aryadi
Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia
title Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia
title_full Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia
title_fullStr Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia
title_full_unstemmed Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia
title_short Evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia
title_sort evaluation of bone marrow-derived mesenchymal stem cell quality from patients with congenital pseudoarthrosis of the tibia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199809/
https://www.ncbi.nlm.nih.gov/pubmed/30352605
http://dx.doi.org/10.1186/s13018-018-0977-9
work_keys_str_mv AT dilogoismailhadisoebroto evaluationofbonemarrowderivedmesenchymalstemcellqualityfrompatientswithcongenitalpseudoarthrosisofthetibia
AT mujadidfajar evaluationofbonemarrowderivedmesenchymalstemcellqualityfrompatientswithcongenitalpseudoarthrosisofthetibia
AT nurhayatiretnowahyu evaluationofbonemarrowderivedmesenchymalstemcellqualityfrompatientswithcongenitalpseudoarthrosisofthetibia
AT kurniawanaryadi evaluationofbonemarrowderivedmesenchymalstemcellqualityfrompatientswithcongenitalpseudoarthrosisofthetibia