Cargando…

Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey

INTRODUCTION: Bovine Norovirus (BoNeV) which has been confirmed in Asia, America, and Europe, seems to be distributed worldwide, even though only reported from a number of countries. Bovine noroviruses are predominantly detected in diarrhoeic animals rather than neboviruses. The study reveals the im...

Descripción completa

Detalles Bibliográficos
Autores principales: Turan, Turhan, Işıdan, Hakan, Atasoy, Mustafa Ozan, Irehan, Bünyamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200295/
https://www.ncbi.nlm.nih.gov/pubmed/30364905
http://dx.doi.org/10.2478/jvetres-2018-0021
_version_ 1783365310615650304
author Turan, Turhan
Işıdan, Hakan
Atasoy, Mustafa Ozan
Irehan, Bünyamin
author_facet Turan, Turhan
Işıdan, Hakan
Atasoy, Mustafa Ozan
Irehan, Bünyamin
author_sort Turan, Turhan
collection PubMed
description INTRODUCTION: Bovine Norovirus (BoNeV) which has been confirmed in Asia, America, and Europe, seems to be distributed worldwide, even though only reported from a number of countries. Bovine noroviruses are predominantly detected in diarrhoeic animals rather than neboviruses. The study reveals the importance of noro- and neboviruses in early age diarrhoea of calves. MATERIAL AND METHODS: A total of 127 stool samples were collected from three provinces located in the central region of Turkey. Samples were subjected to nucleic acid isolation and reverse transcription and polymerase chain reaction (PCR). Positive samples were sequenced and analysed. RESULTS: According to PCR, five samples (3.93%) were found to be positive for bovine norovirus while 32 (25.19%) samples were found to be positive for bovine nebovirus. Phylogenetic analysis indicated that the novel Turkish norovirus strains were found to be of genotype III.2 and all novel neboviruses were substituted under Nebraska-like strains. CONCLUSION: Although predominantly bovine noroviruses are detected worldwide, the study indicated that bovine neboviruses were more prevalent in the studied area. We suggest that bovine neboviruses are more frequently responsible for calf diarrhoea than supposed by virologists. This is also the first report of neboviruses other than Kirklareli virus which is distantly related to neboviruses detected in Turkey.
format Online
Article
Text
id pubmed-6200295
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Sciendo
record_format MEDLINE/PubMed
spelling pubmed-62002952018-10-25 Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey Turan, Turhan Işıdan, Hakan Atasoy, Mustafa Ozan Irehan, Bünyamin J Vet Res Research Article INTRODUCTION: Bovine Norovirus (BoNeV) which has been confirmed in Asia, America, and Europe, seems to be distributed worldwide, even though only reported from a number of countries. Bovine noroviruses are predominantly detected in diarrhoeic animals rather than neboviruses. The study reveals the importance of noro- and neboviruses in early age diarrhoea of calves. MATERIAL AND METHODS: A total of 127 stool samples were collected from three provinces located in the central region of Turkey. Samples were subjected to nucleic acid isolation and reverse transcription and polymerase chain reaction (PCR). Positive samples were sequenced and analysed. RESULTS: According to PCR, five samples (3.93%) were found to be positive for bovine norovirus while 32 (25.19%) samples were found to be positive for bovine nebovirus. Phylogenetic analysis indicated that the novel Turkish norovirus strains were found to be of genotype III.2 and all novel neboviruses were substituted under Nebraska-like strains. CONCLUSION: Although predominantly bovine noroviruses are detected worldwide, the study indicated that bovine neboviruses were more prevalent in the studied area. We suggest that bovine neboviruses are more frequently responsible for calf diarrhoea than supposed by virologists. This is also the first report of neboviruses other than Kirklareli virus which is distantly related to neboviruses detected in Turkey. Sciendo 2018-10-24 /pmc/articles/PMC6200295/ /pubmed/30364905 http://dx.doi.org/10.2478/jvetres-2018-0021 Text en © 2018 Turhan Turan et al., published by Sciendo http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
spellingShingle Research Article
Turan, Turhan
Işıdan, Hakan
Atasoy, Mustafa Ozan
Irehan, Bünyamin
Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey
title Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey
title_full Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey
title_fullStr Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey
title_full_unstemmed Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey
title_short Detection and Molecular Analysis of Bovine Enteric Norovirus and Nebovirus in Turkey
title_sort detection and molecular analysis of bovine enteric norovirus and nebovirus in turkey
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200295/
https://www.ncbi.nlm.nih.gov/pubmed/30364905
http://dx.doi.org/10.2478/jvetres-2018-0021
work_keys_str_mv AT turanturhan detectionandmolecularanalysisofbovineentericnorovirusandnebovirusinturkey
AT isıdanhakan detectionandmolecularanalysisofbovineentericnorovirusandnebovirusinturkey
AT atasoymustafaozan detectionandmolecularanalysisofbovineentericnorovirusandnebovirusinturkey
AT irehanbunyamin detectionandmolecularanalysisofbovineentericnorovirusandnebovirusinturkey