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OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots
This study was aimed at unravelling the molecular basis of root growth behavior in a drought-tolerant upland rice genotype, Nootripathu. Root tips of Nootripathu were found to possess shorter root caps and a greater number of dividing cells, favoring faster elongation compared to shallow-rooted IR20...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200752/ https://www.ncbi.nlm.nih.gov/pubmed/30356087 http://dx.doi.org/10.1038/s41598-018-34053-y |
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author | Ramanathan, Valarmathi Rahman, Hifzur Subramanian, Saravanan Nallathambi, Jagadeeshselvam Kaliyaperumal, Ashokkumar Manickam, Sudha Ranganathan, Chandrababu Muthurajan, Raveendran |
author_facet | Ramanathan, Valarmathi Rahman, Hifzur Subramanian, Saravanan Nallathambi, Jagadeeshselvam Kaliyaperumal, Ashokkumar Manickam, Sudha Ranganathan, Chandrababu Muthurajan, Raveendran |
author_sort | Ramanathan, Valarmathi |
collection | PubMed |
description | This study was aimed at unravelling the molecular basis of root growth behavior in a drought-tolerant upland rice genotype, Nootripathu. Root tips of Nootripathu were found to possess shorter root caps and a greater number of dividing cells, favoring faster elongation compared to shallow-rooted IR20. Width and length of cortical cells in the roots of rapidly growing Nootripathu were found to be two to three times higher than IR20. Evaluation of shallow-rooted IR20, deep-rooted Nootripathu and their Recombinant Inbred Lines (RILs) for root characteristics revealed the presence of genetic variation for root traits among RILs. 2D-PAGE analysis of proteins in roots of IR20, Nootripathu and bulks of extreme RILs differing in root traits resulted in the identification of proteins co-segregating with root growth behavior and co-localized with QTLs for root traits. A putative candidate gene, OsARD4, encoding an “acireductone dioxygenase” was validated for its role in modulating the root growth pattern through genetic transformation. Transgenic ASD16 rice plants engineered for the overexpression of OsARD4 exhibited root growth characteristics similar to those of Nootripathu, including faster radical emergence, more rapid elongation of primary roots, early initiation of crown/lateral roots, and higher root biomass than the non-transgenic plants. |
format | Online Article Text |
id | pubmed-6200752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62007522018-10-25 OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots Ramanathan, Valarmathi Rahman, Hifzur Subramanian, Saravanan Nallathambi, Jagadeeshselvam Kaliyaperumal, Ashokkumar Manickam, Sudha Ranganathan, Chandrababu Muthurajan, Raveendran Sci Rep Article This study was aimed at unravelling the molecular basis of root growth behavior in a drought-tolerant upland rice genotype, Nootripathu. Root tips of Nootripathu were found to possess shorter root caps and a greater number of dividing cells, favoring faster elongation compared to shallow-rooted IR20. Width and length of cortical cells in the roots of rapidly growing Nootripathu were found to be two to three times higher than IR20. Evaluation of shallow-rooted IR20, deep-rooted Nootripathu and their Recombinant Inbred Lines (RILs) for root characteristics revealed the presence of genetic variation for root traits among RILs. 2D-PAGE analysis of proteins in roots of IR20, Nootripathu and bulks of extreme RILs differing in root traits resulted in the identification of proteins co-segregating with root growth behavior and co-localized with QTLs for root traits. A putative candidate gene, OsARD4, encoding an “acireductone dioxygenase” was validated for its role in modulating the root growth pattern through genetic transformation. Transgenic ASD16 rice plants engineered for the overexpression of OsARD4 exhibited root growth characteristics similar to those of Nootripathu, including faster radical emergence, more rapid elongation of primary roots, early initiation of crown/lateral roots, and higher root biomass than the non-transgenic plants. Nature Publishing Group UK 2018-10-24 /pmc/articles/PMC6200752/ /pubmed/30356087 http://dx.doi.org/10.1038/s41598-018-34053-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ramanathan, Valarmathi Rahman, Hifzur Subramanian, Saravanan Nallathambi, Jagadeeshselvam Kaliyaperumal, Ashokkumar Manickam, Sudha Ranganathan, Chandrababu Muthurajan, Raveendran OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots |
title | OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots |
title_full | OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots |
title_fullStr | OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots |
title_full_unstemmed | OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots |
title_short | OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots |
title_sort | osard4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200752/ https://www.ncbi.nlm.nih.gov/pubmed/30356087 http://dx.doi.org/10.1038/s41598-018-34053-y |
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