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Real-Time Ligand Binding of Fluorescent VEGF-A Isoforms that Discriminate between VEGFR2 and NRP1 in Living Cells

Fluorescent VEGF-A isoforms have been evaluated for their ability to discriminate between VEGFR2 and NRP1 in real-time ligand binding studies in live cells using BRET. To enable this, we synthesized single-site (N-terminal cysteine) labeled versions of VEGF(165)a, VEGF(165)b, and VEGF(121)a. These w...

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Detalles Bibliográficos
Autores principales:	Peach, Chloe J., Kilpatrick, Laura E., Friedman-Ohana, Rachel, Zimmerman, Kris, Robers, Matthew B., Wood, Keith V., Woolard, Jeanette, Hill, Stephen J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cell Press 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200776/ https://www.ncbi.nlm.nih.gov/pubmed/30057299 http://dx.doi.org/10.1016/j.chembiol.2018.06.012

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