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Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology
Distortions of the normal bi-concave disc shape for red blood cells (RBCs) appear in a number of pathologies resulting from defects in cell membrane skeletal architecture, erythrocyte ageing, and mechanical damage. We present here the potential of acoustic cytometry for developing new approaches to...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200821/ https://www.ncbi.nlm.nih.gov/pubmed/30356059 http://dx.doi.org/10.1038/s41598-018-33411-0 |
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author | Rico, Laura G. Juncà, Jordi Ward, Mike D. Bradford, Jolene A. Bardina, Jorge Petriz, Jordi |
author_facet | Rico, Laura G. Juncà, Jordi Ward, Mike D. Bradford, Jolene A. Bardina, Jorge Petriz, Jordi |
author_sort | Rico, Laura G. |
collection | PubMed |
description | Distortions of the normal bi-concave disc shape for red blood cells (RBCs) appear in a number of pathologies resulting from defects in cell membrane skeletal architecture, erythrocyte ageing, and mechanical damage. We present here the potential of acoustic cytometry for developing new approaches to light-scattering based evaluation of red blood cell disorders and of the effects of storage and ageing on changes or damage to RBCs membranes. These approaches could be used to immediately evaluate the quality of erythrocytes prior to blood donation and following transfusion. They could also be applied to studying RBC health in diseases and other pathologies, such as artificial heart valve hemolysis, thermal damage or osmotic fragility. Abnormal distributions of erythrocytes can typically be detected after just 30 to 45 seconds of acquisition time using 1–2 µL starting blood volumes. |
format | Online Article Text |
id | pubmed-6200821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62008212018-10-26 Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology Rico, Laura G. Juncà, Jordi Ward, Mike D. Bradford, Jolene A. Bardina, Jorge Petriz, Jordi Sci Rep Article Distortions of the normal bi-concave disc shape for red blood cells (RBCs) appear in a number of pathologies resulting from defects in cell membrane skeletal architecture, erythrocyte ageing, and mechanical damage. We present here the potential of acoustic cytometry for developing new approaches to light-scattering based evaluation of red blood cell disorders and of the effects of storage and ageing on changes or damage to RBCs membranes. These approaches could be used to immediately evaluate the quality of erythrocytes prior to blood donation and following transfusion. They could also be applied to studying RBC health in diseases and other pathologies, such as artificial heart valve hemolysis, thermal damage or osmotic fragility. Abnormal distributions of erythrocytes can typically be detected after just 30 to 45 seconds of acquisition time using 1–2 µL starting blood volumes. Nature Publishing Group UK 2018-10-24 /pmc/articles/PMC6200821/ /pubmed/30356059 http://dx.doi.org/10.1038/s41598-018-33411-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Rico, Laura G. Juncà, Jordi Ward, Mike D. Bradford, Jolene A. Bardina, Jorge Petriz, Jordi Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology |
title | Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology |
title_full | Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology |
title_fullStr | Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology |
title_full_unstemmed | Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology |
title_short | Acoustophoretic Orientation of Red Blood Cells for Diagnosis of Red Cell Health and Pathology |
title_sort | acoustophoretic orientation of red blood cells for diagnosis of red cell health and pathology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200821/ https://www.ncbi.nlm.nih.gov/pubmed/30356059 http://dx.doi.org/10.1038/s41598-018-33411-0 |
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