Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis

Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Here, 1...

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Autores principales: Carter, Glen P., Ussher, James E., Da Silva, Anders Gonçalves, Baines, Sarah L., Heffernan, Helen, Riley, Thomas V., Broadbent, Roland, van der Linden, Antje, Lee, Jean, Monk, Ian R., Stinear, Timothy P., Howden, Benjamin P., Williamson, Deborah A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201123/
https://www.ncbi.nlm.nih.gov/pubmed/30150477
http://dx.doi.org/10.1128/AAC.00898-18
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author Carter, Glen P.
Ussher, James E.
Da Silva, Anders Gonçalves
Baines, Sarah L.
Heffernan, Helen
Riley, Thomas V.
Broadbent, Roland
van der Linden, Antje
Lee, Jean
Monk, Ian R.
Stinear, Timothy P.
Howden, Benjamin P.
Williamson, Deborah A.
author_facet Carter, Glen P.
Ussher, James E.
Da Silva, Anders Gonçalves
Baines, Sarah L.
Heffernan, Helen
Riley, Thomas V.
Broadbent, Roland
van der Linden, Antje
Lee, Jean
Monk, Ian R.
Stinear, Timothy P.
Howden, Benjamin P.
Williamson, Deborah A.
author_sort Carter, Glen P.
collection PubMed
description Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Here, 122 S. capitis isolates from New Zealand (NZ) underwent whole-genome sequencing (WGS), and these data were supplemented with publicly available S. capitis sequence reads. Phylogenetic and comparative genomic analyses were performed, as were phenotypic assessments of antimicrobial resistance, biofilm formation, and plasmid segregational stability on representative isolates. A distinct lineage of S. capitis was identified in NZ associated with neonates and the NICU environment. Isolates from this lineage produced increased levels of biofilm, displayed higher levels of tolerance to chlorhexidine, and were multidrug resistant. Although similar to globally circulating NICU-associated S. capitis strains at a core-genome level, NZ NICU S. capitis isolates carried a novel stably maintained multidrug-resistant plasmid that was not present in non-NICU isolates. Neonatal blood culture isolates were indistinguishable from environmental S. capitis isolates found on fomites, such as stethoscopes and neonatal incubators, but were generally distinct from those isolates carried by NICU staff. This work implicates the NICU environment as a potential reservoir for neonatal sepsis caused by S. capitis and highlights the capacity of genomics-based tracking and surveillance to inform future hospital infection control practices aimed at containing the spread of this important neonatal pathogen.
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spelling pubmed-62011232018-11-15 Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis Carter, Glen P. Ussher, James E. Da Silva, Anders Gonçalves Baines, Sarah L. Heffernan, Helen Riley, Thomas V. Broadbent, Roland van der Linden, Antje Lee, Jean Monk, Ian R. Stinear, Timothy P. Howden, Benjamin P. Williamson, Deborah A. Antimicrob Agents Chemother Epidemiology and Surveillance Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Here, 122 S. capitis isolates from New Zealand (NZ) underwent whole-genome sequencing (WGS), and these data were supplemented with publicly available S. capitis sequence reads. Phylogenetic and comparative genomic analyses were performed, as were phenotypic assessments of antimicrobial resistance, biofilm formation, and plasmid segregational stability on representative isolates. A distinct lineage of S. capitis was identified in NZ associated with neonates and the NICU environment. Isolates from this lineage produced increased levels of biofilm, displayed higher levels of tolerance to chlorhexidine, and were multidrug resistant. Although similar to globally circulating NICU-associated S. capitis strains at a core-genome level, NZ NICU S. capitis isolates carried a novel stably maintained multidrug-resistant plasmid that was not present in non-NICU isolates. Neonatal blood culture isolates were indistinguishable from environmental S. capitis isolates found on fomites, such as stethoscopes and neonatal incubators, but were generally distinct from those isolates carried by NICU staff. This work implicates the NICU environment as a potential reservoir for neonatal sepsis caused by S. capitis and highlights the capacity of genomics-based tracking and surveillance to inform future hospital infection control practices aimed at containing the spread of this important neonatal pathogen. American Society for Microbiology 2018-10-24 /pmc/articles/PMC6201123/ /pubmed/30150477 http://dx.doi.org/10.1128/AAC.00898-18 Text en Copyright © 2018 Carter et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Epidemiology and Surveillance
Carter, Glen P.
Ussher, James E.
Da Silva, Anders Gonçalves
Baines, Sarah L.
Heffernan, Helen
Riley, Thomas V.
Broadbent, Roland
van der Linden, Antje
Lee, Jean
Monk, Ian R.
Stinear, Timothy P.
Howden, Benjamin P.
Williamson, Deborah A.
Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis
title Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis
title_full Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis
title_fullStr Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis
title_full_unstemmed Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis
title_short Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis
title_sort genomic analysis of multiresistant staphylococcus capitis associated with neonatal sepsis
topic Epidemiology and Surveillance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201123/
https://www.ncbi.nlm.nih.gov/pubmed/30150477
http://dx.doi.org/10.1128/AAC.00898-18
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