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Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations
BACKGROUND: Enteroviruses are common human pathogens occasionally associated with severe disease, notoriously paralytic poliomyelitis caused by poliovirus. Other enterovirus serotypes such as enterovirus A71 and D68 have been linked to severe neurological syndromes. New enterovirus serotypes continu...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201154/ https://www.ncbi.nlm.nih.gov/pubmed/30377626 http://dx.doi.org/10.1093/ofid/ofy250 |
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author | Majumdar, Manasi Sharif, Salmaan Klapsa, Dimitra Wilton, Thomas Alam, Muhammad Masroor Fernandez-Garcia, Maria Dolores Rehman, Lubna Mujtaba, Ghulam McAllister, Gina Harvala, Heli Templeton, Kate Mee, Edward T Asghar, Humayun Ndiaye, Kader Minor, Philip D Martin, Javier |
author_facet | Majumdar, Manasi Sharif, Salmaan Klapsa, Dimitra Wilton, Thomas Alam, Muhammad Masroor Fernandez-Garcia, Maria Dolores Rehman, Lubna Mujtaba, Ghulam McAllister, Gina Harvala, Heli Templeton, Kate Mee, Edward T Asghar, Humayun Ndiaye, Kader Minor, Philip D Martin, Javier |
author_sort | Majumdar, Manasi |
collection | PubMed |
description | BACKGROUND: Enteroviruses are common human pathogens occasionally associated with severe disease, notoriously paralytic poliomyelitis caused by poliovirus. Other enterovirus serotypes such as enterovirus A71 and D68 have been linked to severe neurological syndromes. New enterovirus serotypes continue to emerge, some believed to be derived from nonhuman primates. However, little is known about the circulation patterns of many enterovirus serotypes and, in particular, the detailed enterovirus composition of sewage samples. METHODS: We used a next-generation sequencing approach analyzing reverse transcriptase polymerase chain reaction products synthesized directly from sewage concentrates. RESULTS: We determined whole-capsid genome sequences of multiple enterovirus strains from all 4 A to D species present in environmental samples from the United Kingdom, Senegal, and Pakistan. CONCLUSIONS: Our results indicate complex enterovirus circulation patterns in human populations with differences in serotype composition between samples and evidence of sustained and widespread circulation of many enterovirus serotypes. Our analyses revealed known and divergent enterovirus strains, some of public health relevance and genetically linked to clinical isolates. Enteroviruses identified in sewage included vaccine-derived poliovirus and enterovirus D-68 stains, new enterovirus A71 and coxsackievirus A16 genogroups indigenous to Pakistan, and many strains from rarely reported serotypes. We show how this approach can be used for the early detection of emerging pathogens and to improve our understanding of enterovirus circulation in humans. |
format | Online Article Text |
id | pubmed-6201154 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-62011542018-10-30 Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations Majumdar, Manasi Sharif, Salmaan Klapsa, Dimitra Wilton, Thomas Alam, Muhammad Masroor Fernandez-Garcia, Maria Dolores Rehman, Lubna Mujtaba, Ghulam McAllister, Gina Harvala, Heli Templeton, Kate Mee, Edward T Asghar, Humayun Ndiaye, Kader Minor, Philip D Martin, Javier Open Forum Infect Dis Major Article BACKGROUND: Enteroviruses are common human pathogens occasionally associated with severe disease, notoriously paralytic poliomyelitis caused by poliovirus. Other enterovirus serotypes such as enterovirus A71 and D68 have been linked to severe neurological syndromes. New enterovirus serotypes continue to emerge, some believed to be derived from nonhuman primates. However, little is known about the circulation patterns of many enterovirus serotypes and, in particular, the detailed enterovirus composition of sewage samples. METHODS: We used a next-generation sequencing approach analyzing reverse transcriptase polymerase chain reaction products synthesized directly from sewage concentrates. RESULTS: We determined whole-capsid genome sequences of multiple enterovirus strains from all 4 A to D species present in environmental samples from the United Kingdom, Senegal, and Pakistan. CONCLUSIONS: Our results indicate complex enterovirus circulation patterns in human populations with differences in serotype composition between samples and evidence of sustained and widespread circulation of many enterovirus serotypes. Our analyses revealed known and divergent enterovirus strains, some of public health relevance and genetically linked to clinical isolates. Enteroviruses identified in sewage included vaccine-derived poliovirus and enterovirus D-68 stains, new enterovirus A71 and coxsackievirus A16 genogroups indigenous to Pakistan, and many strains from rarely reported serotypes. We show how this approach can be used for the early detection of emerging pathogens and to improve our understanding of enterovirus circulation in humans. Oxford University Press 2018-10-01 /pmc/articles/PMC6201154/ /pubmed/30377626 http://dx.doi.org/10.1093/ofid/ofy250 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Major Article Majumdar, Manasi Sharif, Salmaan Klapsa, Dimitra Wilton, Thomas Alam, Muhammad Masroor Fernandez-Garcia, Maria Dolores Rehman, Lubna Mujtaba, Ghulam McAllister, Gina Harvala, Heli Templeton, Kate Mee, Edward T Asghar, Humayun Ndiaye, Kader Minor, Philip D Martin, Javier Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations |
title | Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations |
title_full | Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations |
title_fullStr | Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations |
title_full_unstemmed | Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations |
title_short | Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations |
title_sort | environmental surveillance reveals complex enterovirus circulation patterns in human populations |
topic | Major Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201154/ https://www.ncbi.nlm.nih.gov/pubmed/30377626 http://dx.doi.org/10.1093/ofid/ofy250 |
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