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miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation
The treatment of complicated long segment strictures remains to a challenge, and the substitution urethroplasty treatment is often accompanied by subsequent tissue fibrosis and secondary stricture formation. In situ injection of human adipose tissue‐derived stem cells (hADSC) could potential be appl...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201219/ https://www.ncbi.nlm.nih.gov/pubmed/30179296 http://dx.doi.org/10.1111/jcmm.13834 |
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author | Feng, Zongcheng Chen, Hongrun Fu, Taozhu Zhang, Lianfeng Liu, Yushan |
author_facet | Feng, Zongcheng Chen, Hongrun Fu, Taozhu Zhang, Lianfeng Liu, Yushan |
author_sort | Feng, Zongcheng |
collection | PubMed |
description | The treatment of complicated long segment strictures remains to a challenge, and the substitution urethroplasty treatment is often accompanied by subsequent tissue fibrosis and secondary stricture formation. In situ injection of human adipose tissue‐derived stem cells (hADSC) could potential be applied for prevention of urethral fibrosis, but the cells transplantation alone may be insufficient because of the complicated histopathological micro‐environmental changes in the injury site. This study investigated whether miR‐21 modification can improve the therapeutic efficacy of ADSCs against urethral fibrosis to limit urethral stricture recurrence. MiR‐21‐modified ADSCs (miR‐21) were constructed via lentivirus‐mediated transfer of pre‐miR‐21 and GFP reporter gene. In vitro results suggested that miR‐21 modification can increase the angiogenesis genes expression of ADSCs and enhance its anti‐oxidative effects against reactive oxygen species (ROS) damage. In vivo results showed that miR‐21 modification contributes to increased urodynamic parameters and better formation of the epithelium and the muscle layer as compared to ADSCs transplantation alone groups. The results demonstrated that miR‐21 modification in ADSCs could improve urethral wound healing microenvironment, enhance stem cell survival through ROS scavenging and promote the neovascularization via regulating angiogenic genes expression, which eventually increase the ADSCs' therapeutic potential for urethral wound healing. |
format | Online Article Text |
id | pubmed-6201219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62012192018-11-01 miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation Feng, Zongcheng Chen, Hongrun Fu, Taozhu Zhang, Lianfeng Liu, Yushan J Cell Mol Med Original Articles The treatment of complicated long segment strictures remains to a challenge, and the substitution urethroplasty treatment is often accompanied by subsequent tissue fibrosis and secondary stricture formation. In situ injection of human adipose tissue‐derived stem cells (hADSC) could potential be applied for prevention of urethral fibrosis, but the cells transplantation alone may be insufficient because of the complicated histopathological micro‐environmental changes in the injury site. This study investigated whether miR‐21 modification can improve the therapeutic efficacy of ADSCs against urethral fibrosis to limit urethral stricture recurrence. MiR‐21‐modified ADSCs (miR‐21) were constructed via lentivirus‐mediated transfer of pre‐miR‐21 and GFP reporter gene. In vitro results suggested that miR‐21 modification can increase the angiogenesis genes expression of ADSCs and enhance its anti‐oxidative effects against reactive oxygen species (ROS) damage. In vivo results showed that miR‐21 modification contributes to increased urodynamic parameters and better formation of the epithelium and the muscle layer as compared to ADSCs transplantation alone groups. The results demonstrated that miR‐21 modification in ADSCs could improve urethral wound healing microenvironment, enhance stem cell survival through ROS scavenging and promote the neovascularization via regulating angiogenic genes expression, which eventually increase the ADSCs' therapeutic potential for urethral wound healing. John Wiley and Sons Inc. 2018-09-04 2018-11 /pmc/articles/PMC6201219/ /pubmed/30179296 http://dx.doi.org/10.1111/jcmm.13834 Text en © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Feng, Zongcheng Chen, Hongrun Fu, Taozhu Zhang, Lianfeng Liu, Yushan miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation |
title | miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation |
title_full | miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation |
title_fullStr | miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation |
title_full_unstemmed | miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation |
title_short | miR‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation |
title_sort | mir‐21 modification enhances the performance of adipose tissue‐derived mesenchymal stem cells for counteracting urethral stricture formation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201219/ https://www.ncbi.nlm.nih.gov/pubmed/30179296 http://dx.doi.org/10.1111/jcmm.13834 |
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