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Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth
Bacterial isolates obtained from the rhizosphere of Arabidopsis and a plantless compost potting mix was screened for anti-oomycete activity against Phytophthora capsici, Phytophthora citricola, Phytophthora palmivora, and Phytophthora cinnamomi. Three out of 48 isolates exhibited more than 65% inhib...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201231/ https://www.ncbi.nlm.nih.gov/pubmed/30405657 http://dx.doi.org/10.3389/fpls.2018.01502 |
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author | Syed-Ab-Rahman, Sharifah Farhana Carvalhais, Lilia C. Chua, Elvis Xiao, Yawen Wass, Taylor J. Schenk, Peer M. |
author_facet | Syed-Ab-Rahman, Sharifah Farhana Carvalhais, Lilia C. Chua, Elvis Xiao, Yawen Wass, Taylor J. Schenk, Peer M. |
author_sort | Syed-Ab-Rahman, Sharifah Farhana |
collection | PubMed |
description | Bacterial isolates obtained from the rhizosphere of Arabidopsis and a plantless compost potting mix was screened for anti-oomycete activity against Phytophthora capsici, Phytophthora citricola, Phytophthora palmivora, and Phytophthora cinnamomi. Three out of 48 isolates exhibited more than 65% inhibition against all tested Phytophthora species and were selected for further studies. These strains, named UQ154, UQ156, and UQ202, are closely related to Bacillus amyloliquefaciens, Bacillus velezensis, and Acinetobacter sp., respectively, based on 16S rDNA sequence analysis. The isolates were evaluated for their ability to fix nitrogen, solubilize phosphate, as well as for siderophore, indoleacetic acid, cell wall degrading enzymes and biofilm production. Their plant growth promoting activities were evaluated by measuring their effect on the germination percentage, root and shoot length, and seedling vigor of lettuce plants. All of these traits were significantly enhanced in plants grown from seeds inoculated with the isolates compared with control plants. Moreover, bacteria-inoculated P. capsici-infected chili plants exhibited improved productivity based on CO(2) assimilation rates. Both real-time quantitative PCR and disease severity index revealed significant decreases in pathogen load in infected chili root tissues when plants were previously inoculated with the isolates. Biocontrol activity may result from the secretion of diketopiperazines as identified by Gas chromatography-mass spectrometry analysis of bacterial cultures’ extracts. Collectively, this work demonstrates the potential of bacterial isolates to control Phytophthora infection and promote plant growth. They can, therefore be considered as candidate microbial biofertilizers and biopesticides. |
format | Online Article Text |
id | pubmed-6201231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62012312018-11-07 Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth Syed-Ab-Rahman, Sharifah Farhana Carvalhais, Lilia C. Chua, Elvis Xiao, Yawen Wass, Taylor J. Schenk, Peer M. Front Plant Sci Plant Science Bacterial isolates obtained from the rhizosphere of Arabidopsis and a plantless compost potting mix was screened for anti-oomycete activity against Phytophthora capsici, Phytophthora citricola, Phytophthora palmivora, and Phytophthora cinnamomi. Three out of 48 isolates exhibited more than 65% inhibition against all tested Phytophthora species and were selected for further studies. These strains, named UQ154, UQ156, and UQ202, are closely related to Bacillus amyloliquefaciens, Bacillus velezensis, and Acinetobacter sp., respectively, based on 16S rDNA sequence analysis. The isolates were evaluated for their ability to fix nitrogen, solubilize phosphate, as well as for siderophore, indoleacetic acid, cell wall degrading enzymes and biofilm production. Their plant growth promoting activities were evaluated by measuring their effect on the germination percentage, root and shoot length, and seedling vigor of lettuce plants. All of these traits were significantly enhanced in plants grown from seeds inoculated with the isolates compared with control plants. Moreover, bacteria-inoculated P. capsici-infected chili plants exhibited improved productivity based on CO(2) assimilation rates. Both real-time quantitative PCR and disease severity index revealed significant decreases in pathogen load in infected chili root tissues when plants were previously inoculated with the isolates. Biocontrol activity may result from the secretion of diketopiperazines as identified by Gas chromatography-mass spectrometry analysis of bacterial cultures’ extracts. Collectively, this work demonstrates the potential of bacterial isolates to control Phytophthora infection and promote plant growth. They can, therefore be considered as candidate microbial biofertilizers and biopesticides. Frontiers Media S.A. 2018-10-18 /pmc/articles/PMC6201231/ /pubmed/30405657 http://dx.doi.org/10.3389/fpls.2018.01502 Text en Copyright © 2018 Syed-Ab-Rahman, Carvalhais, Chua, Xiao, Wass and Schenk. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Syed-Ab-Rahman, Sharifah Farhana Carvalhais, Lilia C. Chua, Elvis Xiao, Yawen Wass, Taylor J. Schenk, Peer M. Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth |
title | Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth |
title_full | Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth |
title_fullStr | Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth |
title_full_unstemmed | Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth |
title_short | Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth |
title_sort | identification of soil bacterial isolates suppressing different phytophthora spp. and promoting plant growth |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201231/ https://www.ncbi.nlm.nih.gov/pubmed/30405657 http://dx.doi.org/10.3389/fpls.2018.01502 |
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