Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

BACKGROUND: Transposon mutagenesis is highly valuable for bacterial genetic and genomic studies. The transposons are usually delivered into host cells through conjugation or electroporation of a suicide plasmid. However, many bacterial species cannot be efficiently conjugated or transformed for tran...

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Detalles Bibliográficos
Autores principales: Naorem, Santa S., Han, Jin, Zhang, Stephanie Y., Zhang, Junyi, Graham, Lindsey B., Song, Angelou, Smith, Cameron V., Rashid, Fariha, Guo, Huatao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201506/
https://www.ncbi.nlm.nih.gov/pubmed/30355324
http://dx.doi.org/10.1186/s12866-018-1319-0

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