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The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling

BACKGROUND: Excretory-secretory products released by Echinococcus granulosus protoscoleces (EgPSC-ESPs) are well-known to regulate T cell responses. However, their direct influence on the differentiation of B cell subsets remains largely elusive. This study investigated the effects of EgPSC-ESPs on...

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Autores principales: Pan, Wei, Xu, Hui-wen, Hao, Wen-ting, Sun, Fen-fen, Qin, Yan-fang, Hao, Shan-shan, Liu, Hua, Cao, Jian-ping, Shen, Yu-juan, Zheng, Kui-yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201587/
https://www.ncbi.nlm.nih.gov/pubmed/30355335
http://dx.doi.org/10.1186/s12865-018-0267-7
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author Pan, Wei
Xu, Hui-wen
Hao, Wen-ting
Sun, Fen-fen
Qin, Yan-fang
Hao, Shan-shan
Liu, Hua
Cao, Jian-ping
Shen, Yu-juan
Zheng, Kui-yang
author_facet Pan, Wei
Xu, Hui-wen
Hao, Wen-ting
Sun, Fen-fen
Qin, Yan-fang
Hao, Shan-shan
Liu, Hua
Cao, Jian-ping
Shen, Yu-juan
Zheng, Kui-yang
author_sort Pan, Wei
collection PubMed
description BACKGROUND: Excretory-secretory products released by Echinococcus granulosus protoscoleces (EgPSC-ESPs) are well-known to regulate T cell responses. However, their direct influence on the differentiation of B cell subsets remains largely elusive. This study investigated the effects of EgPSC-ESPs on the differentiation of IL-10-producing B cells (B10), and explored the possible role of Toll-like receptor 2 (TLR-2) signaling in this process. RESULTS: In comparison to phosphate buffered saline (PBS), B cells exposed to the excretory–secretory products (ESPs) generated higher percentages of B10 cells, with higher expression of IL-10 mRNA, and larger amount of IL-10 production, which were in a dose dependent way. The mRNA and protein expression of TLR-2 in the ESPs-stimulated B cells were significantly higher than those in PBS, which was consistent to the results in B cells isolated from EgPSC infected mice. Moreover, TLR-2(−/−) B cells in response to ESPs stimulation expressed lower levels of IL-10 mRNA and produced undetectable IL-10 in comparison to those in normal B cells. In addition, Phosphatase and tensin homolog deleted on chromosome ten/AKT/Phosphatidylinositol-3 kinase (PTEN/AKT/PI3K) pathway was activated in ESPs-treated B cells, which was also dependent on TLR-2 signaling. Pam3CSK4, the agonist of TLR-2, could mock the effects of ESPs on the expression of PTEN, AKT and PI3K. CONCLUSION: Overall, this study revealed that TLR-2 signaling was required for B10 induction mediated by EgPSC-ESPs, which might be an immunomodulatory target against the parasite infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12865-018-0267-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-62015872018-10-31 The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling Pan, Wei Xu, Hui-wen Hao, Wen-ting Sun, Fen-fen Qin, Yan-fang Hao, Shan-shan Liu, Hua Cao, Jian-ping Shen, Yu-juan Zheng, Kui-yang BMC Immunol Research Article BACKGROUND: Excretory-secretory products released by Echinococcus granulosus protoscoleces (EgPSC-ESPs) are well-known to regulate T cell responses. However, their direct influence on the differentiation of B cell subsets remains largely elusive. This study investigated the effects of EgPSC-ESPs on the differentiation of IL-10-producing B cells (B10), and explored the possible role of Toll-like receptor 2 (TLR-2) signaling in this process. RESULTS: In comparison to phosphate buffered saline (PBS), B cells exposed to the excretory–secretory products (ESPs) generated higher percentages of B10 cells, with higher expression of IL-10 mRNA, and larger amount of IL-10 production, which were in a dose dependent way. The mRNA and protein expression of TLR-2 in the ESPs-stimulated B cells were significantly higher than those in PBS, which was consistent to the results in B cells isolated from EgPSC infected mice. Moreover, TLR-2(−/−) B cells in response to ESPs stimulation expressed lower levels of IL-10 mRNA and produced undetectable IL-10 in comparison to those in normal B cells. In addition, Phosphatase and tensin homolog deleted on chromosome ten/AKT/Phosphatidylinositol-3 kinase (PTEN/AKT/PI3K) pathway was activated in ESPs-treated B cells, which was also dependent on TLR-2 signaling. Pam3CSK4, the agonist of TLR-2, could mock the effects of ESPs on the expression of PTEN, AKT and PI3K. CONCLUSION: Overall, this study revealed that TLR-2 signaling was required for B10 induction mediated by EgPSC-ESPs, which might be an immunomodulatory target against the parasite infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12865-018-0267-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-10-24 /pmc/articles/PMC6201587/ /pubmed/30355335 http://dx.doi.org/10.1186/s12865-018-0267-7 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Pan, Wei
Xu, Hui-wen
Hao, Wen-ting
Sun, Fen-fen
Qin, Yan-fang
Hao, Shan-shan
Liu, Hua
Cao, Jian-ping
Shen, Yu-juan
Zheng, Kui-yang
The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling
title The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling
title_full The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling
title_fullStr The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling
title_full_unstemmed The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling
title_short The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling
title_sort excretory-secretory products of echinococcus granulosus protoscoleces stimulated il-10 production in b cells via tlr-2 signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201587/
https://www.ncbi.nlm.nih.gov/pubmed/30355335
http://dx.doi.org/10.1186/s12865-018-0267-7
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