A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activa...

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Detalles Bibliográficos
Autores principales: Sim, Yong-Kyun, Jung, Heetae, Kim, Su Hyun, Park, Jung-Woo, Park, Woo-Jin, Jun, Chul-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201817/
https://www.ncbi.nlm.nih.gov/pubmed/30430001
http://dx.doi.org/10.1039/c8sc02565g
Descripción
Sumario:A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.

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