A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution

A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activa...

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Detalles Bibliográficos
Autores principales: Sim, Yong-Kyun, Jung, Heetae, Kim, Su Hyun, Park, Jung-Woo, Park, Woo-Jin, Jun, Chul-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201817/
https://www.ncbi.nlm.nih.gov/pubmed/30430001
http://dx.doi.org/10.1039/c8sc02565g
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author Sim, Yong-Kyun
Jung, Heetae
Kim, Su Hyun
Park, Jung-Woo
Park, Woo-Jin
Jun, Chul-Ho
author_facet Sim, Yong-Kyun
Jung, Heetae
Kim, Su Hyun
Park, Jung-Woo
Park, Woo-Jin
Jun, Chul-Ho
author_sort Sim, Yong-Kyun
collection PubMed
description A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.
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spelling pubmed-62018172018-11-14 A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution Sim, Yong-Kyun Jung, Heetae Kim, Su Hyun Park, Jung-Woo Park, Woo-Jin Jun, Chul-Ho Chem Sci Chemistry A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule. Royal Society of Chemistry 2018-08-24 /pmc/articles/PMC6201817/ /pubmed/30430001 http://dx.doi.org/10.1039/c8sc02565g Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Sim, Yong-Kyun
Jung, Heetae
Kim, Su Hyun
Park, Jung-Woo
Park, Woo-Jin
Jun, Chul-Ho
A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution
title A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution
title_full A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution
title_fullStr A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution
title_full_unstemmed A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution
title_short A one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution
title_sort one-step method for covalent bond immobilization of biomolecules on silica operated in aqueous solution
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201817/
https://www.ncbi.nlm.nih.gov/pubmed/30430001
http://dx.doi.org/10.1039/c8sc02565g
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