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In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells
microRNA (miRNA)-26a-loaded liposomes were prepared in the present study for effective treatment of leukemia. The results demonstrated that miRNA-26a reduced the viability of chronic lymphocytic leukemia (CLL) cells in a concentration-dependent manner. Cells treated with miRNA-26a-loaded liposomes e...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202071/ https://www.ncbi.nlm.nih.gov/pubmed/30320374 http://dx.doi.org/10.3892/ijmm.2018.3925 |
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author | Li, Jing Sun, Chang-Kui |
author_facet | Li, Jing Sun, Chang-Kui |
author_sort | Li, Jing |
collection | PubMed |
description | microRNA (miRNA)-26a-loaded liposomes were prepared in the present study for effective treatment of leukemia. The results demonstrated that miRNA-26a reduced the viability of chronic lymphocytic leukemia (CLL) cells in a concentration-dependent manner. Cells treated with miRNA-26a-loaded liposomes exhibited increased rates of apoptosis, as determined by flow cytometry and Hoechst 33342 staining. Western blot analysis revealed an increased apoptotic effect of miRNA-26a-loaded liposomes compared with control. Treatment with these liposomes resulted in significant downregulation of the expression of the miRNA-26a target genes, myeloid cell leukemia 1 and cyclin-dependent kinase 6. Taken together, the results of the present study indicate that miRNA-26a exerts apoptosis-inducing and anticancer effects on leukemia cells, suggesting therapeutic potential. This approach may be possible to extrapolate to other neoplasms, including lymphomas and acute myeloid leukemia. |
format | Online Article Text |
id | pubmed-6202071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-62020712018-11-07 In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells Li, Jing Sun, Chang-Kui Int J Mol Med Articles microRNA (miRNA)-26a-loaded liposomes were prepared in the present study for effective treatment of leukemia. The results demonstrated that miRNA-26a reduced the viability of chronic lymphocytic leukemia (CLL) cells in a concentration-dependent manner. Cells treated with miRNA-26a-loaded liposomes exhibited increased rates of apoptosis, as determined by flow cytometry and Hoechst 33342 staining. Western blot analysis revealed an increased apoptotic effect of miRNA-26a-loaded liposomes compared with control. Treatment with these liposomes resulted in significant downregulation of the expression of the miRNA-26a target genes, myeloid cell leukemia 1 and cyclin-dependent kinase 6. Taken together, the results of the present study indicate that miRNA-26a exerts apoptosis-inducing and anticancer effects on leukemia cells, suggesting therapeutic potential. This approach may be possible to extrapolate to other neoplasms, including lymphomas and acute myeloid leukemia. D.A. Spandidos 2018-12 2018-10-10 /pmc/articles/PMC6202071/ /pubmed/30320374 http://dx.doi.org/10.3892/ijmm.2018.3925 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Jing Sun, Chang-Kui In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells |
title | In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells |
title_full | In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells |
title_fullStr | In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells |
title_full_unstemmed | In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells |
title_short | In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells |
title_sort | in vitro analysis of microrna-26a in chronic lymphocytic leukemia cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202071/ https://www.ncbi.nlm.nih.gov/pubmed/30320374 http://dx.doi.org/10.3892/ijmm.2018.3925 |
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