C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2

Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstra...

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Detalles Bibliográficos
Autores principales: Gao, Xin D., Tu, Li-Chun, Mir, Aamir, Rodriguez, Tomás, Ding, Yuehe, Leszyk, John, Dekker, Job, Shaffer, Scott A., Zhu, Lihua Julie, Wolfe, Scot A., Sontheimer, Erik J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202229/
https://www.ncbi.nlm.nih.gov/pubmed/29735996
http://dx.doi.org/10.1038/s41592-018-0006-2
Descripción
Sumario:Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear biology.

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