C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2

Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstra...

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Autores principales: Gao, Xin D., Tu, Li-Chun, Mir, Aamir, Rodriguez, Tomás, Ding, Yuehe, Leszyk, John, Dekker, Job, Shaffer, Scott A., Zhu, Lihua Julie, Wolfe, Scot A., Sontheimer, Erik J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202229/
https://www.ncbi.nlm.nih.gov/pubmed/29735996
http://dx.doi.org/10.1038/s41592-018-0006-2
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author Gao, Xin D.
Tu, Li-Chun
Mir, Aamir
Rodriguez, Tomás
Ding, Yuehe
Leszyk, John
Dekker, Job
Shaffer, Scott A.
Zhu, Lihua Julie
Wolfe, Scot A.
Sontheimer, Erik J.
author_facet Gao, Xin D.
Tu, Li-Chun
Mir, Aamir
Rodriguez, Tomás
Ding, Yuehe
Leszyk, John
Dekker, Job
Shaffer, Scott A.
Zhu, Lihua Julie
Wolfe, Scot A.
Sontheimer, Erik J.
author_sort Gao, Xin D.
collection PubMed
description Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear biology.
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spelling pubmed-62022292018-11-07 C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2 Gao, Xin D. Tu, Li-Chun Mir, Aamir Rodriguez, Tomás Ding, Yuehe Leszyk, John Dekker, Job Shaffer, Scott A. Zhu, Lihua Julie Wolfe, Scot A. Sontheimer, Erik J. Nat Methods Article Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear biology. 2018-05-07 2018-06 /pmc/articles/PMC6202229/ /pubmed/29735996 http://dx.doi.org/10.1038/s41592-018-0006-2 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms Reprints and permissions information is available online at http://www.nature.com/reprints/index.html
spellingShingle Article
Gao, Xin D.
Tu, Li-Chun
Mir, Aamir
Rodriguez, Tomás
Ding, Yuehe
Leszyk, John
Dekker, Job
Shaffer, Scott A.
Zhu, Lihua Julie
Wolfe, Scot A.
Sontheimer, Erik J.
C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
title C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
title_full C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
title_fullStr C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
title_full_unstemmed C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
title_short C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
title_sort c-berst: defining subnuclear proteomic landscapes at genomic elements with dcas9-apex2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202229/
https://www.ncbi.nlm.nih.gov/pubmed/29735996
http://dx.doi.org/10.1038/s41592-018-0006-2
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