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C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
Mapping proteomic composition at distinct genomic loci in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the rapid, unbiased mapping of proteomes near defined genomic loci, as demonstra...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202229/ https://www.ncbi.nlm.nih.gov/pubmed/29735996 http://dx.doi.org/10.1038/s41592-018-0006-2 |