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Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting
Recent advances in the field of Tissue Engineering allowed to control the three-dimensional organization of engineered constructs. Cell pattern imaging and in vivo follow-up remain a major hurdle in in situ bioprinting onto deep tissues. Magnetic Resonance Imaging (MRI) associated with Micron-sized...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202323/ https://www.ncbi.nlm.nih.gov/pubmed/30361490 http://dx.doi.org/10.1038/s41598-018-34226-9 |
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author | Kérourédan, Olivia Ribot, Emeline Julie Fricain, Jean-Christophe Devillard, Raphaël Miraux, Sylvain |
author_facet | Kérourédan, Olivia Ribot, Emeline Julie Fricain, Jean-Christophe Devillard, Raphaël Miraux, Sylvain |
author_sort | Kérourédan, Olivia |
collection | PubMed |
description | Recent advances in the field of Tissue Engineering allowed to control the three-dimensional organization of engineered constructs. Cell pattern imaging and in vivo follow-up remain a major hurdle in in situ bioprinting onto deep tissues. Magnetic Resonance Imaging (MRI) associated with Micron-sized superParamagnetic Iron Oxide (MPIO) particles constitutes a non-invasive method for tracking cells in vivo. To date, no studies have utilized Cellular MRI as a tool to follow cell patterns obtained via bioprinting technologies. Laser-Assisted Bioprinting (LAB) has been increasingly recognized as a new and exciting addition to the bioprinting’s arsenal, due to its rapidity, precision and ability to print viable cells. This non-contact technology has been successfully used in recent in vivo applications. The aim of this study was to assess the methodology of tracking MPIO-labeled stem cells using MRI after organizing them by Laser-Assisted Bioprinting. Optimal MPIO concentrations for tracking bioprinted cells were determined. Accuracy of printed patterns was compared using MRI and confocal microscopy. Cell densities within the patterns and MRI signals were correlated. MRI enabled to detect cell patterns after in situ bioprinting onto a mouse calvarial defect. Results demonstrate that MRI combined with MPIO cell labeling is a valuable technique to track bioprinted cells in vitro and in animal models. |
format | Online Article Text |
id | pubmed-6202323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62023232018-10-29 Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting Kérourédan, Olivia Ribot, Emeline Julie Fricain, Jean-Christophe Devillard, Raphaël Miraux, Sylvain Sci Rep Article Recent advances in the field of Tissue Engineering allowed to control the three-dimensional organization of engineered constructs. Cell pattern imaging and in vivo follow-up remain a major hurdle in in situ bioprinting onto deep tissues. Magnetic Resonance Imaging (MRI) associated with Micron-sized superParamagnetic Iron Oxide (MPIO) particles constitutes a non-invasive method for tracking cells in vivo. To date, no studies have utilized Cellular MRI as a tool to follow cell patterns obtained via bioprinting technologies. Laser-Assisted Bioprinting (LAB) has been increasingly recognized as a new and exciting addition to the bioprinting’s arsenal, due to its rapidity, precision and ability to print viable cells. This non-contact technology has been successfully used in recent in vivo applications. The aim of this study was to assess the methodology of tracking MPIO-labeled stem cells using MRI after organizing them by Laser-Assisted Bioprinting. Optimal MPIO concentrations for tracking bioprinted cells were determined. Accuracy of printed patterns was compared using MRI and confocal microscopy. Cell densities within the patterns and MRI signals were correlated. MRI enabled to detect cell patterns after in situ bioprinting onto a mouse calvarial defect. Results demonstrate that MRI combined with MPIO cell labeling is a valuable technique to track bioprinted cells in vitro and in animal models. Nature Publishing Group UK 2018-10-25 /pmc/articles/PMC6202323/ /pubmed/30361490 http://dx.doi.org/10.1038/s41598-018-34226-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kérourédan, Olivia Ribot, Emeline Julie Fricain, Jean-Christophe Devillard, Raphaël Miraux, Sylvain Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting |
title | Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting |
title_full | Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting |
title_fullStr | Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting |
title_full_unstemmed | Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting |
title_short | Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting |
title_sort | magnetic resonance imaging for tracking cellular patterns obtained by laser-assisted bioprinting |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202323/ https://www.ncbi.nlm.nih.gov/pubmed/30361490 http://dx.doi.org/10.1038/s41598-018-34226-9 |
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