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The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process
Although N-acyl-L-homoserine lactone (AHL) based quorum sensing (QS) phenomenon has been observed in mature anaerobic granules, the biological role of AHL-based QS system in anaerobic granulation process remains unexplored. For the first time, a long-term anaerobic bioreactor was operated for 168 da...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202354/ https://www.ncbi.nlm.nih.gov/pubmed/30361560 http://dx.doi.org/10.1038/s41598-018-34183-3 |
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author | Ma, Haijun Ma, Sijia Hu, Haidong Ding, Lili Ren, Hongqiang |
author_facet | Ma, Haijun Ma, Sijia Hu, Haidong Ding, Lili Ren, Hongqiang |
author_sort | Ma, Haijun |
collection | PubMed |
description | Although N-acyl-L-homoserine lactone (AHL) based quorum sensing (QS) phenomenon has been observed in mature anaerobic granules, the biological role of AHL-based QS system in anaerobic granulation process remains unexplored. For the first time, a long-term anaerobic bioreactor was operated for 168 days to investigate the biological role of AHL in the granulation process which was divided into three phases (phase I: floccular, phase II: granulation, phase III: maturation). Two different AHLs including C8-HSL and C10-HSL were characterized at nanogram levels. The AHL level was elevated over 20-fold and strongly positively correlated with extracellular polymeric substances (EPS) production and sludge particle size during phase I-II. Exogenous addition of AHL to the floccular sludge also resulted in significantly increased EPS production. Metadata analysis suggested that the granulation process was accompanied by an increase in the abundance of QS-relevant microorganisms. The strong relationships (R > 0.9233, p < 0.01) among AHL concentrations, EPS (except loosely bound EPS), granulation and community variation indicated that AHL-mediated QS played an important role in coordinating community level behaviors associated with granulation, potentially through the regulation of EPS production and composition. This study gives a deep insight into the underlying QS-relevant mechanism of anaerobic granulation process. |
format | Online Article Text |
id | pubmed-6202354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62023542018-10-29 The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process Ma, Haijun Ma, Sijia Hu, Haidong Ding, Lili Ren, Hongqiang Sci Rep Article Although N-acyl-L-homoserine lactone (AHL) based quorum sensing (QS) phenomenon has been observed in mature anaerobic granules, the biological role of AHL-based QS system in anaerobic granulation process remains unexplored. For the first time, a long-term anaerobic bioreactor was operated for 168 days to investigate the biological role of AHL in the granulation process which was divided into three phases (phase I: floccular, phase II: granulation, phase III: maturation). Two different AHLs including C8-HSL and C10-HSL were characterized at nanogram levels. The AHL level was elevated over 20-fold and strongly positively correlated with extracellular polymeric substances (EPS) production and sludge particle size during phase I-II. Exogenous addition of AHL to the floccular sludge also resulted in significantly increased EPS production. Metadata analysis suggested that the granulation process was accompanied by an increase in the abundance of QS-relevant microorganisms. The strong relationships (R > 0.9233, p < 0.01) among AHL concentrations, EPS (except loosely bound EPS), granulation and community variation indicated that AHL-mediated QS played an important role in coordinating community level behaviors associated with granulation, potentially through the regulation of EPS production and composition. This study gives a deep insight into the underlying QS-relevant mechanism of anaerobic granulation process. Nature Publishing Group UK 2018-10-25 /pmc/articles/PMC6202354/ /pubmed/30361560 http://dx.doi.org/10.1038/s41598-018-34183-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ma, Haijun Ma, Sijia Hu, Haidong Ding, Lili Ren, Hongqiang The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process |
title | The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process |
title_full | The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process |
title_fullStr | The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process |
title_full_unstemmed | The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process |
title_short | The biological role of N-acyl-homoserine lactone-based quorum sensing (QS) in EPS production and microbial community assembly during anaerobic granulation process |
title_sort | biological role of n-acyl-homoserine lactone-based quorum sensing (qs) in eps production and microbial community assembly during anaerobic granulation process |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202354/ https://www.ncbi.nlm.nih.gov/pubmed/30361560 http://dx.doi.org/10.1038/s41598-018-34183-3 |
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