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Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting

The shape of cellular membranes is highly regulated by a set of conserved mechanisms that can be manipulated by bacterial pathogens to infect cells. Remodeling of the plasma membrane of endothelial cells by the bacterium Neisseria meningitidis is thought to be essential during the blood phase of men...

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Autores principales: Charles-Orszag, Arthur, Tsai, Feng-Ching, Bonazzi, Daria, Manriquez, Valeria, Sachse, Martin, Mallet, Adeline, Salles, Audrey, Melican, Keira, Staneva, Ralitza, Bertin, Aurélie, Millien, Corinne, Goussard, Sylvie, Lafaye, Pierre, Shorte, Spencer, Piel, Matthieu, Krijnse-Locker, Jacomine, Brochard-Wyart, Françoise, Bassereau, Patricia, Duménil, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202395/
https://www.ncbi.nlm.nih.gov/pubmed/30361638
http://dx.doi.org/10.1038/s41467-018-06948-x
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author Charles-Orszag, Arthur
Tsai, Feng-Ching
Bonazzi, Daria
Manriquez, Valeria
Sachse, Martin
Mallet, Adeline
Salles, Audrey
Melican, Keira
Staneva, Ralitza
Bertin, Aurélie
Millien, Corinne
Goussard, Sylvie
Lafaye, Pierre
Shorte, Spencer
Piel, Matthieu
Krijnse-Locker, Jacomine
Brochard-Wyart, Françoise
Bassereau, Patricia
Duménil, Guillaume
author_facet Charles-Orszag, Arthur
Tsai, Feng-Ching
Bonazzi, Daria
Manriquez, Valeria
Sachse, Martin
Mallet, Adeline
Salles, Audrey
Melican, Keira
Staneva, Ralitza
Bertin, Aurélie
Millien, Corinne
Goussard, Sylvie
Lafaye, Pierre
Shorte, Spencer
Piel, Matthieu
Krijnse-Locker, Jacomine
Brochard-Wyart, Françoise
Bassereau, Patricia
Duménil, Guillaume
author_sort Charles-Orszag, Arthur
collection PubMed
description The shape of cellular membranes is highly regulated by a set of conserved mechanisms that can be manipulated by bacterial pathogens to infect cells. Remodeling of the plasma membrane of endothelial cells by the bacterium Neisseria meningitidis is thought to be essential during the blood phase of meningococcal infection, but the underlying mechanisms are unclear. Here we show that plasma membrane remodeling occurs independently of F-actin, along meningococcal type IV pili fibers, by a physical mechanism that we term ‘one-dimensional’ membrane wetting. We provide a theoretical model that describes the physical basis of one-dimensional wetting and show that this mechanism occurs in model membranes interacting with nanofibers, and in human cells interacting with extracellular matrix meshworks. We propose one-dimensional wetting as a new general principle driving the interaction of cells with their environment at the nanoscale that is diverted by meningococci during infection.
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spelling pubmed-62023952018-10-29 Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting Charles-Orszag, Arthur Tsai, Feng-Ching Bonazzi, Daria Manriquez, Valeria Sachse, Martin Mallet, Adeline Salles, Audrey Melican, Keira Staneva, Ralitza Bertin, Aurélie Millien, Corinne Goussard, Sylvie Lafaye, Pierre Shorte, Spencer Piel, Matthieu Krijnse-Locker, Jacomine Brochard-Wyart, Françoise Bassereau, Patricia Duménil, Guillaume Nat Commun Article The shape of cellular membranes is highly regulated by a set of conserved mechanisms that can be manipulated by bacterial pathogens to infect cells. Remodeling of the plasma membrane of endothelial cells by the bacterium Neisseria meningitidis is thought to be essential during the blood phase of meningococcal infection, but the underlying mechanisms are unclear. Here we show that plasma membrane remodeling occurs independently of F-actin, along meningococcal type IV pili fibers, by a physical mechanism that we term ‘one-dimensional’ membrane wetting. We provide a theoretical model that describes the physical basis of one-dimensional wetting and show that this mechanism occurs in model membranes interacting with nanofibers, and in human cells interacting with extracellular matrix meshworks. We propose one-dimensional wetting as a new general principle driving the interaction of cells with their environment at the nanoscale that is diverted by meningococci during infection. Nature Publishing Group UK 2018-10-25 /pmc/articles/PMC6202395/ /pubmed/30361638 http://dx.doi.org/10.1038/s41467-018-06948-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Charles-Orszag, Arthur
Tsai, Feng-Ching
Bonazzi, Daria
Manriquez, Valeria
Sachse, Martin
Mallet, Adeline
Salles, Audrey
Melican, Keira
Staneva, Ralitza
Bertin, Aurélie
Millien, Corinne
Goussard, Sylvie
Lafaye, Pierre
Shorte, Spencer
Piel, Matthieu
Krijnse-Locker, Jacomine
Brochard-Wyart, Françoise
Bassereau, Patricia
Duménil, Guillaume
Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting
title Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting
title_full Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting
title_fullStr Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting
title_full_unstemmed Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting
title_short Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting
title_sort adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202395/
https://www.ncbi.nlm.nih.gov/pubmed/30361638
http://dx.doi.org/10.1038/s41467-018-06948-x
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