Cargando…
Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor
Infection diagnosis and antibiotic susceptibility testing (AST) are time-consuming and often laborious clinical practices. This paper presents a microwave-microfluidic biosensor for rapid, contactless and non-invasive device for testing the concentration and growth of Escherichia Coli (E. Coli) in m...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202403/ https://www.ncbi.nlm.nih.gov/pubmed/30361480 http://dx.doi.org/10.1038/s41598-018-34001-w |
_version_ | 1783365673101033472 |
---|---|
author | Narang, Rakesh Mohammadi, Sevda Ashani, Mehdi Mohammadi Sadabadi, Hamid Hejazi, Hossein Zarifi, Mohammad Hossein Sanati-Nezhad, Amir |
author_facet | Narang, Rakesh Mohammadi, Sevda Ashani, Mehdi Mohammadi Sadabadi, Hamid Hejazi, Hossein Zarifi, Mohammad Hossein Sanati-Nezhad, Amir |
author_sort | Narang, Rakesh |
collection | PubMed |
description | Infection diagnosis and antibiotic susceptibility testing (AST) are time-consuming and often laborious clinical practices. This paper presents a microwave-microfluidic biosensor for rapid, contactless and non-invasive device for testing the concentration and growth of Escherichia Coli (E. Coli) in medium solutions of different pH to increase the efficacy of clinical microbiology practices. The thin layer interface between the microfluidic channel and the microwave resonator significantly enhanced the detection sensitivity. The microfluidic chip, fabricated using standard soft lithography, was injected with bacterial samples and incorporated with a microwave microstrip ring resonator sensor with an operation frequency of 2.5 GHz and initial quality factor of 83 for detecting the concentration and growth of bacteria. The resonator had a coupling gap area on of 1.5 × 1.5 mm(2) as of its sensitive region. The presence of different concentrations of bacteria in different pH solutions were detected via screening the changes in resonant amplitude and frequency responses of the microwave system. The sensor device demonstrated near immediate response to changes in the concentration of bacteria and maximum sensitivity of 3.4 MHz compared to a logarithm value of bacteria concentration. The minimum prepared optical transparency of bacteria was tested at an OD(600) value of 0.003. The sensor’s resonant frequency and amplitude parameters were utilized to monitor bacteria growth during a 500-minute time frame, which demonstrated a stable response with respect to detecting the bacterial proliferation. A highly linear response was demonstrated for detecting bacteria concentration at various pH values. The growth of bacteria analyzed over the resonator showed an exponential growth curve with respect to time and concurred with the lag-log-stationary-death model of cell growth. This biosensor is one step forward to automate the complex AST workflow of clinical microbiology laboratories for rapid and automated detection of bacteria as well as screening the bacteria proliferation in response to antibiotics. |
format | Online Article Text |
id | pubmed-6202403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62024032018-10-29 Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor Narang, Rakesh Mohammadi, Sevda Ashani, Mehdi Mohammadi Sadabadi, Hamid Hejazi, Hossein Zarifi, Mohammad Hossein Sanati-Nezhad, Amir Sci Rep Article Infection diagnosis and antibiotic susceptibility testing (AST) are time-consuming and often laborious clinical practices. This paper presents a microwave-microfluidic biosensor for rapid, contactless and non-invasive device for testing the concentration and growth of Escherichia Coli (E. Coli) in medium solutions of different pH to increase the efficacy of clinical microbiology practices. The thin layer interface between the microfluidic channel and the microwave resonator significantly enhanced the detection sensitivity. The microfluidic chip, fabricated using standard soft lithography, was injected with bacterial samples and incorporated with a microwave microstrip ring resonator sensor with an operation frequency of 2.5 GHz and initial quality factor of 83 for detecting the concentration and growth of bacteria. The resonator had a coupling gap area on of 1.5 × 1.5 mm(2) as of its sensitive region. The presence of different concentrations of bacteria in different pH solutions were detected via screening the changes in resonant amplitude and frequency responses of the microwave system. The sensor device demonstrated near immediate response to changes in the concentration of bacteria and maximum sensitivity of 3.4 MHz compared to a logarithm value of bacteria concentration. The minimum prepared optical transparency of bacteria was tested at an OD(600) value of 0.003. The sensor’s resonant frequency and amplitude parameters were utilized to monitor bacteria growth during a 500-minute time frame, which demonstrated a stable response with respect to detecting the bacterial proliferation. A highly linear response was demonstrated for detecting bacteria concentration at various pH values. The growth of bacteria analyzed over the resonator showed an exponential growth curve with respect to time and concurred with the lag-log-stationary-death model of cell growth. This biosensor is one step forward to automate the complex AST workflow of clinical microbiology laboratories for rapid and automated detection of bacteria as well as screening the bacteria proliferation in response to antibiotics. Nature Publishing Group UK 2018-10-25 /pmc/articles/PMC6202403/ /pubmed/30361480 http://dx.doi.org/10.1038/s41598-018-34001-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Narang, Rakesh Mohammadi, Sevda Ashani, Mehdi Mohammadi Sadabadi, Hamid Hejazi, Hossein Zarifi, Mohammad Hossein Sanati-Nezhad, Amir Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor |
title | Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor |
title_full | Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor |
title_fullStr | Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor |
title_full_unstemmed | Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor |
title_short | Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor |
title_sort | sensitive, real-time and non-intrusive detection of concentration and growth of pathogenic bacteria using microfluidic-microwave ring resonator biosensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202403/ https://www.ncbi.nlm.nih.gov/pubmed/30361480 http://dx.doi.org/10.1038/s41598-018-34001-w |
work_keys_str_mv | AT narangrakesh sensitiverealtimeandnonintrusivedetectionofconcentrationandgrowthofpathogenicbacteriausingmicrofluidicmicrowaveringresonatorbiosensor AT mohammadisevda sensitiverealtimeandnonintrusivedetectionofconcentrationandgrowthofpathogenicbacteriausingmicrofluidicmicrowaveringresonatorbiosensor AT ashanimehdimohammadi sensitiverealtimeandnonintrusivedetectionofconcentrationandgrowthofpathogenicbacteriausingmicrofluidicmicrowaveringresonatorbiosensor AT sadabadihamid sensitiverealtimeandnonintrusivedetectionofconcentrationandgrowthofpathogenicbacteriausingmicrofluidicmicrowaveringresonatorbiosensor AT hejazihossein sensitiverealtimeandnonintrusivedetectionofconcentrationandgrowthofpathogenicbacteriausingmicrofluidicmicrowaveringresonatorbiosensor AT zarifimohammadhossein sensitiverealtimeandnonintrusivedetectionofconcentrationandgrowthofpathogenicbacteriausingmicrofluidicmicrowaveringresonatorbiosensor AT sanatinezhadamir sensitiverealtimeandnonintrusivedetectionofconcentrationandgrowthofpathogenicbacteriausingmicrofluidicmicrowaveringresonatorbiosensor |