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Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer

Glycogen Synthase Kinase-3β (GSK-3β), a serine/threonine protein kinase, has been implicated as a potential therapeutic target in human cancer. The objective of the present study was to evaluate aberrant expression of GSK-3β as a potential biomarker in human breast and head and neck cancers. Nuclear...

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Autores principales: Ugolkov, Andrey V., Matsangou, Maria, Taxter, Timothy J., O'Halloran, Thomas V., Cryns, Vincent L., Giles, Francis J., Mazar, Andrew P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202539/
https://www.ncbi.nlm.nih.gov/pubmed/30405781
http://dx.doi.org/10.3892/ol.2018.9483
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author Ugolkov, Andrey V.
Matsangou, Maria
Taxter, Timothy J.
O'Halloran, Thomas V.
Cryns, Vincent L.
Giles, Francis J.
Mazar, Andrew P.
author_facet Ugolkov, Andrey V.
Matsangou, Maria
Taxter, Timothy J.
O'Halloran, Thomas V.
Cryns, Vincent L.
Giles, Francis J.
Mazar, Andrew P.
author_sort Ugolkov, Andrey V.
collection PubMed
description Glycogen Synthase Kinase-3β (GSK-3β), a serine/threonine protein kinase, has been implicated as a potential therapeutic target in human cancer. The objective of the present study was to evaluate aberrant expression of GSK-3β as a potential biomarker in human breast and head and neck cancers. Nuclear/cytosolic fractionation, immunoblotting and immunohistochemical staining was used to study the expression of GSK-3β in human breast and head and neck cancer. Aberrant nuclear accumulation of GSK-3β in five human breast cancer cell lines was demonstrated and in 89/128 (70%) human breast carcinomas, whereas no detectable expression of GSK-3β was found in benign breast tissue. Nuclear GSK-3β expression was associated with HER-2 positive tumors (P=0.02) and non-triple negative breast carcinomas (P=0.0001), although nuclear GSK-3β was observed in some samples across all breast cancer subtypes. Aberrant nuclear expression of GSK-3β was found in 11/15 (73%) squamous cell head and neck carcinomas, whereas weak or no detectable expression of GSK-3β was found in benign salivary gland and other benign head and neck tissues. These results support the hypothesis that aberrant nuclear GSK-3β may represent a potential target for the clinical treatment of human breast and squamous cell carcinoma.
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spelling pubmed-62025392018-11-07 Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer Ugolkov, Andrey V. Matsangou, Maria Taxter, Timothy J. O'Halloran, Thomas V. Cryns, Vincent L. Giles, Francis J. Mazar, Andrew P. Oncol Lett Articles Glycogen Synthase Kinase-3β (GSK-3β), a serine/threonine protein kinase, has been implicated as a potential therapeutic target in human cancer. The objective of the present study was to evaluate aberrant expression of GSK-3β as a potential biomarker in human breast and head and neck cancers. Nuclear/cytosolic fractionation, immunoblotting and immunohistochemical staining was used to study the expression of GSK-3β in human breast and head and neck cancer. Aberrant nuclear accumulation of GSK-3β in five human breast cancer cell lines was demonstrated and in 89/128 (70%) human breast carcinomas, whereas no detectable expression of GSK-3β was found in benign breast tissue. Nuclear GSK-3β expression was associated with HER-2 positive tumors (P=0.02) and non-triple negative breast carcinomas (P=0.0001), although nuclear GSK-3β was observed in some samples across all breast cancer subtypes. Aberrant nuclear expression of GSK-3β was found in 11/15 (73%) squamous cell head and neck carcinomas, whereas weak or no detectable expression of GSK-3β was found in benign salivary gland and other benign head and neck tissues. These results support the hypothesis that aberrant nuclear GSK-3β may represent a potential target for the clinical treatment of human breast and squamous cell carcinoma. D.A. Spandidos 2018-11 2018-09-21 /pmc/articles/PMC6202539/ /pubmed/30405781 http://dx.doi.org/10.3892/ol.2018.9483 Text en Copyright: © Ugolkov et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ugolkov, Andrey V.
Matsangou, Maria
Taxter, Timothy J.
O'Halloran, Thomas V.
Cryns, Vincent L.
Giles, Francis J.
Mazar, Andrew P.
Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer
title Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer
title_full Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer
title_fullStr Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer
title_full_unstemmed Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer
title_short Aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer
title_sort aberrant expression of glycogen synthase kinase-3β in human breast and head and neck cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202539/
https://www.ncbi.nlm.nih.gov/pubmed/30405781
http://dx.doi.org/10.3892/ol.2018.9483
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