Cargando…
Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters
[Image: see text] Inorganic polyphosphate (polyP) is an often-overlooked biopolymer of phosphate residues present in living cells. PolyP is associated with many essential biological roles. Despite interest in polyP’s function, most studies have been limited to extracellular or isolated protein exper...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2018
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202642/ https://www.ncbi.nlm.nih.gov/pubmed/30410977 http://dx.doi.org/10.1021/acscentsci.8b00470 |
_version_ | 1783365721656393728 |
---|---|
author | Fernandes-Cunha, Gabriella M. McKinlay, Colin J. Vargas, Jessica R. Jessen, Henning J. Waymouth, Robert M. Wender, Paul A. |
author_facet | Fernandes-Cunha, Gabriella M. McKinlay, Colin J. Vargas, Jessica R. Jessen, Henning J. Waymouth, Robert M. Wender, Paul A. |
author_sort | Fernandes-Cunha, Gabriella M. |
collection | PubMed |
description | [Image: see text] Inorganic polyphosphate (polyP) is an often-overlooked biopolymer of phosphate residues present in living cells. PolyP is associated with many essential biological roles. Despite interest in polyP’s function, most studies have been limited to extracellular or isolated protein experiments, as polyanionic polyP does not traverse the nonpolar membrane of cells. To address this problem, we developed a robust, readily employed method for polyP delivery using guanidinium-rich oligocarbonate transporters that electrostatically complex polyPs of multiple lengths, forming discrete nanoparticles that are resistant to phosphatase degradation and that readily enter multiple cell types. Fluorescently labeled polyPs have been monitored over time for subcellular localization and release from the transporter, with control over release rates achieved by modulating the transporter identity and the charge ratio of the electrostatic complexes. This general approach to polyP delivery enables the study of intracellular polyP signaling in a variety of applications. |
format | Online Article Text |
id | pubmed-6202642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-62026422018-11-08 Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters Fernandes-Cunha, Gabriella M. McKinlay, Colin J. Vargas, Jessica R. Jessen, Henning J. Waymouth, Robert M. Wender, Paul A. ACS Cent Sci [Image: see text] Inorganic polyphosphate (polyP) is an often-overlooked biopolymer of phosphate residues present in living cells. PolyP is associated with many essential biological roles. Despite interest in polyP’s function, most studies have been limited to extracellular or isolated protein experiments, as polyanionic polyP does not traverse the nonpolar membrane of cells. To address this problem, we developed a robust, readily employed method for polyP delivery using guanidinium-rich oligocarbonate transporters that electrostatically complex polyPs of multiple lengths, forming discrete nanoparticles that are resistant to phosphatase degradation and that readily enter multiple cell types. Fluorescently labeled polyPs have been monitored over time for subcellular localization and release from the transporter, with control over release rates achieved by modulating the transporter identity and the charge ratio of the electrostatic complexes. This general approach to polyP delivery enables the study of intracellular polyP signaling in a variety of applications. American Chemical Society 2018-09-26 2018-10-24 /pmc/articles/PMC6202642/ /pubmed/30410977 http://dx.doi.org/10.1021/acscentsci.8b00470 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Fernandes-Cunha, Gabriella M. McKinlay, Colin J. Vargas, Jessica R. Jessen, Henning J. Waymouth, Robert M. Wender, Paul A. Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters |
title | Delivery of Inorganic Polyphosphate into Cells Using
Amphipathic Oligocarbonate Transporters |
title_full | Delivery of Inorganic Polyphosphate into Cells Using
Amphipathic Oligocarbonate Transporters |
title_fullStr | Delivery of Inorganic Polyphosphate into Cells Using
Amphipathic Oligocarbonate Transporters |
title_full_unstemmed | Delivery of Inorganic Polyphosphate into Cells Using
Amphipathic Oligocarbonate Transporters |
title_short | Delivery of Inorganic Polyphosphate into Cells Using
Amphipathic Oligocarbonate Transporters |
title_sort | delivery of inorganic polyphosphate into cells using
amphipathic oligocarbonate transporters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202642/ https://www.ncbi.nlm.nih.gov/pubmed/30410977 http://dx.doi.org/10.1021/acscentsci.8b00470 |
work_keys_str_mv | AT fernandescunhagabriellam deliveryofinorganicpolyphosphateintocellsusingamphipathicoligocarbonatetransporters AT mckinlaycolinj deliveryofinorganicpolyphosphateintocellsusingamphipathicoligocarbonatetransporters AT vargasjessicar deliveryofinorganicpolyphosphateintocellsusingamphipathicoligocarbonatetransporters AT jessenhenningj deliveryofinorganicpolyphosphateintocellsusingamphipathicoligocarbonatetransporters AT waymouthrobertm deliveryofinorganicpolyphosphateintocellsusingamphipathicoligocarbonatetransporters AT wenderpaula deliveryofinorganicpolyphosphateintocellsusingamphipathicoligocarbonatetransporters |