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Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters

[Image: see text] Inorganic polyphosphate (polyP) is an often-overlooked biopolymer of phosphate residues present in living cells. PolyP is associated with many essential biological roles. Despite interest in polyP’s function, most studies have been limited to extracellular or isolated protein exper...

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Autores principales: Fernandes-Cunha, Gabriella M., McKinlay, Colin J., Vargas, Jessica R., Jessen, Henning J., Waymouth, Robert M., Wender, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202642/
https://www.ncbi.nlm.nih.gov/pubmed/30410977
http://dx.doi.org/10.1021/acscentsci.8b00470
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author Fernandes-Cunha, Gabriella M.
McKinlay, Colin J.
Vargas, Jessica R.
Jessen, Henning J.
Waymouth, Robert M.
Wender, Paul A.
author_facet Fernandes-Cunha, Gabriella M.
McKinlay, Colin J.
Vargas, Jessica R.
Jessen, Henning J.
Waymouth, Robert M.
Wender, Paul A.
author_sort Fernandes-Cunha, Gabriella M.
collection PubMed
description [Image: see text] Inorganic polyphosphate (polyP) is an often-overlooked biopolymer of phosphate residues present in living cells. PolyP is associated with many essential biological roles. Despite interest in polyP’s function, most studies have been limited to extracellular or isolated protein experiments, as polyanionic polyP does not traverse the nonpolar membrane of cells. To address this problem, we developed a robust, readily employed method for polyP delivery using guanidinium-rich oligocarbonate transporters that electrostatically complex polyPs of multiple lengths, forming discrete nanoparticles that are resistant to phosphatase degradation and that readily enter multiple cell types. Fluorescently labeled polyPs have been monitored over time for subcellular localization and release from the transporter, with control over release rates achieved by modulating the transporter identity and the charge ratio of the electrostatic complexes. This general approach to polyP delivery enables the study of intracellular polyP signaling in a variety of applications.
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spelling pubmed-62026422018-11-08 Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters Fernandes-Cunha, Gabriella M. McKinlay, Colin J. Vargas, Jessica R. Jessen, Henning J. Waymouth, Robert M. Wender, Paul A. ACS Cent Sci [Image: see text] Inorganic polyphosphate (polyP) is an often-overlooked biopolymer of phosphate residues present in living cells. PolyP is associated with many essential biological roles. Despite interest in polyP’s function, most studies have been limited to extracellular or isolated protein experiments, as polyanionic polyP does not traverse the nonpolar membrane of cells. To address this problem, we developed a robust, readily employed method for polyP delivery using guanidinium-rich oligocarbonate transporters that electrostatically complex polyPs of multiple lengths, forming discrete nanoparticles that are resistant to phosphatase degradation and that readily enter multiple cell types. Fluorescently labeled polyPs have been monitored over time for subcellular localization and release from the transporter, with control over release rates achieved by modulating the transporter identity and the charge ratio of the electrostatic complexes. This general approach to polyP delivery enables the study of intracellular polyP signaling in a variety of applications. American Chemical Society 2018-09-26 2018-10-24 /pmc/articles/PMC6202642/ /pubmed/30410977 http://dx.doi.org/10.1021/acscentsci.8b00470 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Fernandes-Cunha, Gabriella M.
McKinlay, Colin J.
Vargas, Jessica R.
Jessen, Henning J.
Waymouth, Robert M.
Wender, Paul A.
Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters
title Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters
title_full Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters
title_fullStr Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters
title_full_unstemmed Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters
title_short Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters
title_sort delivery of inorganic polyphosphate into cells using amphipathic oligocarbonate transporters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202642/
https://www.ncbi.nlm.nih.gov/pubmed/30410977
http://dx.doi.org/10.1021/acscentsci.8b00470
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