CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters

BACKGROUND: Recent experimental efforts of CRISPR-Cas9 systems have shown that off-target binding and cleavage are a concern for the system and that this is highly dependent on the selected guide RNA (gRNA) design. Computational predictions of off-targets have been proposed as an attractive and more...

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Detalles Bibliográficos
Autores principales: Alkan, Ferhat, Wenzel, Anne, Anthon, Christian, Havgaard, Jakob Hull, Gorodkin, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203265/
https://www.ncbi.nlm.nih.gov/pubmed/30367669
http://dx.doi.org/10.1186/s13059-018-1534-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203265/
https://www.ncbi.nlm.nih.gov/pubmed/30367669
http://dx.doi.org/10.1186/s13059-018-1534-x

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