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Defining the viability of tardigrades with a molecular sensor related to death

The design of experimental protocols that use animal models to assess the impact of a stress on a population or to determine the life span expectancy impact can be time-consuming due to the need for direct observations of dead and living animals. These experiments are usually based on the detectable...

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Autores principales: Richaud, Myriam, Galas, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203378/
https://www.ncbi.nlm.nih.gov/pubmed/30365540
http://dx.doi.org/10.1371/journal.pone.0206444
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author Richaud, Myriam
Galas, Simon
author_facet Richaud, Myriam
Galas, Simon
author_sort Richaud, Myriam
collection PubMed
description The design of experimental protocols that use animal models to assess the impact of a stress on a population or to determine the life span expectancy impact can be time-consuming due to the need for direct observations of dead and living animals. These experiments are usually based on the detectable activity of animals such as food intake or mobility and can sometimes produce either under- or overestimated results. The tardigrade Hypsibius exemplaris is an emerging model for the evolutionary biology of the tardigrade phylum because of its convenient laboratory breeding and the recent introduction of new molecular tools. In this report, we describe the use of a new fluorescent dye that can specifically stain dead tardigrades. Furthermore, we also monitored the absence of a toxic side effect of the death-linked fluorescent dye on tardigrade populations. Finally, we conclude that tardigrade experiments that require survival counting of the Hypsibius exemplaris species can be greatly improved by using this technique in order to limit underestimation of alive animals.
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spelling pubmed-62033782018-11-19 Defining the viability of tardigrades with a molecular sensor related to death Richaud, Myriam Galas, Simon PLoS One Research Article The design of experimental protocols that use animal models to assess the impact of a stress on a population or to determine the life span expectancy impact can be time-consuming due to the need for direct observations of dead and living animals. These experiments are usually based on the detectable activity of animals such as food intake or mobility and can sometimes produce either under- or overestimated results. The tardigrade Hypsibius exemplaris is an emerging model for the evolutionary biology of the tardigrade phylum because of its convenient laboratory breeding and the recent introduction of new molecular tools. In this report, we describe the use of a new fluorescent dye that can specifically stain dead tardigrades. Furthermore, we also monitored the absence of a toxic side effect of the death-linked fluorescent dye on tardigrade populations. Finally, we conclude that tardigrade experiments that require survival counting of the Hypsibius exemplaris species can be greatly improved by using this technique in order to limit underestimation of alive animals. Public Library of Science 2018-10-26 /pmc/articles/PMC6203378/ /pubmed/30365540 http://dx.doi.org/10.1371/journal.pone.0206444 Text en © 2018 Richaud, Galas http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Richaud, Myriam
Galas, Simon
Defining the viability of tardigrades with a molecular sensor related to death
title Defining the viability of tardigrades with a molecular sensor related to death
title_full Defining the viability of tardigrades with a molecular sensor related to death
title_fullStr Defining the viability of tardigrades with a molecular sensor related to death
title_full_unstemmed Defining the viability of tardigrades with a molecular sensor related to death
title_short Defining the viability of tardigrades with a molecular sensor related to death
title_sort defining the viability of tardigrades with a molecular sensor related to death
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203378/
https://www.ncbi.nlm.nih.gov/pubmed/30365540
http://dx.doi.org/10.1371/journal.pone.0206444
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