NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas

The function of the NAD(P)H oxidases (NOXs) family member NOX4 is to generate reactive oxygen species (ROS), however, the molecular function of NOX4 has not been fully studied and waiting to be clarified. To elucidate the function of endogenous Nox4 in human thyroid carcinomas, papillomatosis thyroi...

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Autores principales: Tang, Ping, Dang, Hao, Huang, Jie, Xu, Tao, Yuan, Ping, Hu, Jun, Sheng, Jian-feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203707/
https://www.ncbi.nlm.nih.gov/pubmed/30367082
http://dx.doi.org/10.1038/s41598-018-34154-8
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author Tang, Ping
Dang, Hao
Huang, Jie
Xu, Tao
Yuan, Ping
Hu, Jun
Sheng, Jian-feng
author_facet Tang, Ping
Dang, Hao
Huang, Jie
Xu, Tao
Yuan, Ping
Hu, Jun
Sheng, Jian-feng
author_sort Tang, Ping
collection PubMed
description The function of the NAD(P)H oxidases (NOXs) family member NOX4 is to generate reactive oxygen species (ROS), however, the molecular function of NOX4 has not been fully studied and waiting to be clarified. To elucidate the function of endogenous Nox4 in human thyroid carcinomas, papillomatosis thyroid cancer cells were used to study the cell growth by knocking down the expression of NOX4 and knocking out its functional partner p22phox/CYBA. As a result, the increasement of mitochondrial ROS(mROS) was abolished due to both knockdown of NOX4 and p22phox knockout in hypoxia, which destabilized HIF1α decreasing glycolysis and retarded cell growth. These data suggests that Nox4 is potent oncotarget due to its role in regulating glycolysis through mROS-HIF1α pathway, thereby mediating proliferation in thyroid carcinomas.
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spelling pubmed-62037072018-10-31 NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas Tang, Ping Dang, Hao Huang, Jie Xu, Tao Yuan, Ping Hu, Jun Sheng, Jian-feng Sci Rep Article The function of the NAD(P)H oxidases (NOXs) family member NOX4 is to generate reactive oxygen species (ROS), however, the molecular function of NOX4 has not been fully studied and waiting to be clarified. To elucidate the function of endogenous Nox4 in human thyroid carcinomas, papillomatosis thyroid cancer cells were used to study the cell growth by knocking down the expression of NOX4 and knocking out its functional partner p22phox/CYBA. As a result, the increasement of mitochondrial ROS(mROS) was abolished due to both knockdown of NOX4 and p22phox knockout in hypoxia, which destabilized HIF1α decreasing glycolysis and retarded cell growth. These data suggests that Nox4 is potent oncotarget due to its role in regulating glycolysis through mROS-HIF1α pathway, thereby mediating proliferation in thyroid carcinomas. Nature Publishing Group UK 2018-10-26 /pmc/articles/PMC6203707/ /pubmed/30367082 http://dx.doi.org/10.1038/s41598-018-34154-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tang, Ping
Dang, Hao
Huang, Jie
Xu, Tao
Yuan, Ping
Hu, Jun
Sheng, Jian-feng
NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_full NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_fullStr NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_full_unstemmed NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_short NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_sort nadph oxidase nox4 is a glycolytic regulator through mros-hif1α axis in thyroid carcinomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203707/
https://www.ncbi.nlm.nih.gov/pubmed/30367082
http://dx.doi.org/10.1038/s41598-018-34154-8
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