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Species diversity of drifting fish eggs in the Yangtze River using molecular identification

The dam constructions greatly changed the hydrologic conditions in the Yangtze River, and then significantly affected the spawning activities of indigenous river fish. Monitoring the species composition of drifting eggs during spawning season is important for protection issues. In this study, we hav...

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Autores principales: Liu, Mingdian, Wang, Dengqiang, Gao, Lei, Tian, Huiwu, Liu, Shaoping, Chen, Daqing, Duan, Xinbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203937/
https://www.ncbi.nlm.nih.gov/pubmed/30386702
http://dx.doi.org/10.7717/peerj.5807
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author Liu, Mingdian
Wang, Dengqiang
Gao, Lei
Tian, Huiwu
Liu, Shaoping
Chen, Daqing
Duan, Xinbin
author_facet Liu, Mingdian
Wang, Dengqiang
Gao, Lei
Tian, Huiwu
Liu, Shaoping
Chen, Daqing
Duan, Xinbin
author_sort Liu, Mingdian
collection PubMed
description The dam constructions greatly changed the hydrologic conditions in the Yangtze River, and then significantly affected the spawning activities of indigenous river fish. Monitoring the species composition of drifting eggs during spawning season is important for protection issues. In this study, we have sampled drifting fish eggs in nine locations from 2014 to 2016. Eggs were identified using the mitochondrial cyt b gene sequence. A total of 7,933 fish eggs were sequenced successfully and blasted into the NCBI database. Thirty-nine fish species were identified, and were assigned to four families and two orders. Approximately 64% of the species identified, and 67% of the eggs, were classified in the Family Cyprinidae. Abundance and Shannon–Wiener diversity index of species were higher in the main river than in tributaries of the river. However, tributaries may be important spawning grounds for some fish species. The Jaccard’s similarity index and river-way distances among sampled stations were negatively correlated suggesting the environment shapes species composition in the sampled spawning grounds. These results showed that mitochondrial DNA sequence is a powerful and effective tool for fish egg identification in Yangtze River and these data are useful for conservation efforts.
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spelling pubmed-62039372018-10-31 Species diversity of drifting fish eggs in the Yangtze River using molecular identification Liu, Mingdian Wang, Dengqiang Gao, Lei Tian, Huiwu Liu, Shaoping Chen, Daqing Duan, Xinbin PeerJ Aquaculture, Fisheries and Fish Science The dam constructions greatly changed the hydrologic conditions in the Yangtze River, and then significantly affected the spawning activities of indigenous river fish. Monitoring the species composition of drifting eggs during spawning season is important for protection issues. In this study, we have sampled drifting fish eggs in nine locations from 2014 to 2016. Eggs were identified using the mitochondrial cyt b gene sequence. A total of 7,933 fish eggs were sequenced successfully and blasted into the NCBI database. Thirty-nine fish species were identified, and were assigned to four families and two orders. Approximately 64% of the species identified, and 67% of the eggs, were classified in the Family Cyprinidae. Abundance and Shannon–Wiener diversity index of species were higher in the main river than in tributaries of the river. However, tributaries may be important spawning grounds for some fish species. The Jaccard’s similarity index and river-way distances among sampled stations were negatively correlated suggesting the environment shapes species composition in the sampled spawning grounds. These results showed that mitochondrial DNA sequence is a powerful and effective tool for fish egg identification in Yangtze River and these data are useful for conservation efforts. PeerJ Inc. 2018-10-24 /pmc/articles/PMC6203937/ /pubmed/30386702 http://dx.doi.org/10.7717/peerj.5807 Text en ©2018 Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Liu, Mingdian
Wang, Dengqiang
Gao, Lei
Tian, Huiwu
Liu, Shaoping
Chen, Daqing
Duan, Xinbin
Species diversity of drifting fish eggs in the Yangtze River using molecular identification
title Species diversity of drifting fish eggs in the Yangtze River using molecular identification
title_full Species diversity of drifting fish eggs in the Yangtze River using molecular identification
title_fullStr Species diversity of drifting fish eggs in the Yangtze River using molecular identification
title_full_unstemmed Species diversity of drifting fish eggs in the Yangtze River using molecular identification
title_short Species diversity of drifting fish eggs in the Yangtze River using molecular identification
title_sort species diversity of drifting fish eggs in the yangtze river using molecular identification
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203937/
https://www.ncbi.nlm.nih.gov/pubmed/30386702
http://dx.doi.org/10.7717/peerj.5807
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