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Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress
BACKGROUND: Heat stress is a severe environmental stress that affects plant growth and reduces yield. Bax inhibitor-1 (BI-1) is a cytoprotective protein that is involved in the response to biotic and abiotic stresses. The Arabidopsis (Arabidopsis thaliana) BI-1 mutants atbi1–1 and atbi1–2 are hypers...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204060/ https://www.ncbi.nlm.nih.gov/pubmed/30367612 http://dx.doi.org/10.1186/s12870-018-1485-0 |
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author | Lu, Pan-Pan Zheng, Wei-Jun Wang, Chang-Tao Shi, Wen-Yan Fu, Jin-Dong Chen, Ming Chen, Jun Zhou, Yong-Bin Xi, Ya-Jun Xu, Zhao-Shi |
author_facet | Lu, Pan-Pan Zheng, Wei-Jun Wang, Chang-Tao Shi, Wen-Yan Fu, Jin-Dong Chen, Ming Chen, Jun Zhou, Yong-Bin Xi, Ya-Jun Xu, Zhao-Shi |
author_sort | Lu, Pan-Pan |
collection | PubMed |
description | BACKGROUND: Heat stress is a severe environmental stress that affects plant growth and reduces yield. Bax inhibitor-1 (BI-1) is a cytoprotective protein that is involved in the response to biotic and abiotic stresses. The Arabidopsis (Arabidopsis thaliana) BI-1 mutants atbi1–1 and atbi1–2 are hypersensitive to heat stress, and AtBI-1 overexpression rescues thermotolerance deficiency in atbi1 plants. Nevertheless, the mechanism of BI-1 in plant thermotolerance is still unclear. RESULTS: We identified a wheat (Triticum aestivum L.) BI-1 gene, TaBI-1.1, which was highly upregulated in an RNA sequencing (RNA-seq) analysis of heat-treated wheat. The upregulation of TaBI-1.1 under heat stress was further demonstrated by real time quantitative PCR (qRT-PCR) and β-glucuronidase (GUS) staining. Compared with the wild type Col-0, the atbi1–2 mutant is hypersensitive to heat stress, and constitutive expression of TaBI-1.1 in atbi1–2 (35S::TaBI-1.1/ atbi1–2) rescued the deficiency of atbi1–2 under heat stress. Furthermore, we identified TaFKBP62 as a TaBI-1.1-interacting protein that co-localized with TaBI-1.1 on the endoplasmic reticulum (ER) membrane and enhanced heat stress tolerance. Additionally, HSFA2, HSFB1, ROF1, HSP17.4B, HSP17.6A, HSP17.8, HSP70B, and HSP90.1 expression levels were suppressed in atbi1–2 plants under heat stress. In contrast, 35S::TaBI-1.1/atbi1–2 relieved the inhibitory effect of AtBI-1 loss of function. CONCLUSIONS: TaBI-1.1 interacted with TaFKBP62 and co-localized with TaFKBP62 on the ER membrane. Both TaBI-1.1 and AtBI-1 regulated the expression of heat-responsive genes and were conserved in plant thermotolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1485-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6204060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62040602018-11-01 Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress Lu, Pan-Pan Zheng, Wei-Jun Wang, Chang-Tao Shi, Wen-Yan Fu, Jin-Dong Chen, Ming Chen, Jun Zhou, Yong-Bin Xi, Ya-Jun Xu, Zhao-Shi BMC Plant Biol Research Article BACKGROUND: Heat stress is a severe environmental stress that affects plant growth and reduces yield. Bax inhibitor-1 (BI-1) is a cytoprotective protein that is involved in the response to biotic and abiotic stresses. The Arabidopsis (Arabidopsis thaliana) BI-1 mutants atbi1–1 and atbi1–2 are hypersensitive to heat stress, and AtBI-1 overexpression rescues thermotolerance deficiency in atbi1 plants. Nevertheless, the mechanism of BI-1 in plant thermotolerance is still unclear. RESULTS: We identified a wheat (Triticum aestivum L.) BI-1 gene, TaBI-1.1, which was highly upregulated in an RNA sequencing (RNA-seq) analysis of heat-treated wheat. The upregulation of TaBI-1.1 under heat stress was further demonstrated by real time quantitative PCR (qRT-PCR) and β-glucuronidase (GUS) staining. Compared with the wild type Col-0, the atbi1–2 mutant is hypersensitive to heat stress, and constitutive expression of TaBI-1.1 in atbi1–2 (35S::TaBI-1.1/ atbi1–2) rescued the deficiency of atbi1–2 under heat stress. Furthermore, we identified TaFKBP62 as a TaBI-1.1-interacting protein that co-localized with TaBI-1.1 on the endoplasmic reticulum (ER) membrane and enhanced heat stress tolerance. Additionally, HSFA2, HSFB1, ROF1, HSP17.4B, HSP17.6A, HSP17.8, HSP70B, and HSP90.1 expression levels were suppressed in atbi1–2 plants under heat stress. In contrast, 35S::TaBI-1.1/atbi1–2 relieved the inhibitory effect of AtBI-1 loss of function. CONCLUSIONS: TaBI-1.1 interacted with TaFKBP62 and co-localized with TaFKBP62 on the ER membrane. Both TaBI-1.1 and AtBI-1 regulated the expression of heat-responsive genes and were conserved in plant thermotolerance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1485-0) contains supplementary material, which is available to authorized users. BioMed Central 2018-10-26 /pmc/articles/PMC6204060/ /pubmed/30367612 http://dx.doi.org/10.1186/s12870-018-1485-0 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Lu, Pan-Pan Zheng, Wei-Jun Wang, Chang-Tao Shi, Wen-Yan Fu, Jin-Dong Chen, Ming Chen, Jun Zhou, Yong-Bin Xi, Ya-Jun Xu, Zhao-Shi Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress |
title | Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress |
title_full | Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress |
title_fullStr | Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress |
title_full_unstemmed | Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress |
title_short | Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress |
title_sort | wheat bax inhibitor-1 interacts with tafkbp62 and mediates response to heat stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204060/ https://www.ncbi.nlm.nih.gov/pubmed/30367612 http://dx.doi.org/10.1186/s12870-018-1485-0 |
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