Cargando…

A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy

A digital hologram-optimizing method was proposed to improve the imaging quality of dual-wavelength digital holographic microscopy (DDHM) by reducing the phase noise level. In our previous work, phase noise reduction was achieved by dual-wavelength digital image-plane holographic microscopy (DDIPHM)...

Descripción completa

Detalles Bibliográficos
Autores principales: Zeng, Yanan, Lu, Junsheng, Chang, Xinyu, Liu, Yuan, Hu, Xiaodong, Su, Kangyan, Chen, Xiayu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204173/
https://www.ncbi.nlm.nih.gov/pubmed/30405871
http://dx.doi.org/10.1155/2018/4582590
_version_ 1783366002012061696
author Zeng, Yanan
Lu, Junsheng
Chang, Xinyu
Liu, Yuan
Hu, Xiaodong
Su, Kangyan
Chen, Xiayu
author_facet Zeng, Yanan
Lu, Junsheng
Chang, Xinyu
Liu, Yuan
Hu, Xiaodong
Su, Kangyan
Chen, Xiayu
author_sort Zeng, Yanan
collection PubMed
description A digital hologram-optimizing method was proposed to improve the imaging quality of dual-wavelength digital holographic microscopy (DDHM) by reducing the phase noise level. In our previous work, phase noise reduction was achieved by dual-wavelength digital image-plane holographic microscopy (DDIPHM). In the optimization method in this paper, the phase noise was further reduced by enhancing the real-image term and suppressing effects of the zero-order term in the frequency spectrum of a digital hologram. Practically, the carrier frequency of the real-image term has the correspondence with interference fringes in the hologram. Mathematically, the first order intrinsic mode function (IMF1) in bidimensional empirical mode decomposition (BEMD) has similar characteristics to the grayscale values of ideal interference fringes. Therefore, with the combination of DDIPHM and BEMD, by utilizing the characteristics of IMF1, the digital hologram was optimized with purified interference fringes, enhancing the real-image term simultaneously. Finally, the validity of the proposed method was verified by experimental results on a microstep.
format Online
Article
Text
id pubmed-6204173
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-62041732018-11-07 A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy Zeng, Yanan Lu, Junsheng Chang, Xinyu Liu, Yuan Hu, Xiaodong Su, Kangyan Chen, Xiayu Scanning Research Article A digital hologram-optimizing method was proposed to improve the imaging quality of dual-wavelength digital holographic microscopy (DDHM) by reducing the phase noise level. In our previous work, phase noise reduction was achieved by dual-wavelength digital image-plane holographic microscopy (DDIPHM). In the optimization method in this paper, the phase noise was further reduced by enhancing the real-image term and suppressing effects of the zero-order term in the frequency spectrum of a digital hologram. Practically, the carrier frequency of the real-image term has the correspondence with interference fringes in the hologram. Mathematically, the first order intrinsic mode function (IMF1) in bidimensional empirical mode decomposition (BEMD) has similar characteristics to the grayscale values of ideal interference fringes. Therefore, with the combination of DDIPHM and BEMD, by utilizing the characteristics of IMF1, the digital hologram was optimized with purified interference fringes, enhancing the real-image term simultaneously. Finally, the validity of the proposed method was verified by experimental results on a microstep. Hindawi 2018-10-14 /pmc/articles/PMC6204173/ /pubmed/30405871 http://dx.doi.org/10.1155/2018/4582590 Text en Copyright © 2018 Yanan Zeng et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zeng, Yanan
Lu, Junsheng
Chang, Xinyu
Liu, Yuan
Hu, Xiaodong
Su, Kangyan
Chen, Xiayu
A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy
title A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy
title_full A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy
title_fullStr A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy
title_full_unstemmed A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy
title_short A Method to Improve the Imaging Quality in Dual-Wavelength Digital Holographic Microscopy
title_sort method to improve the imaging quality in dual-wavelength digital holographic microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204173/
https://www.ncbi.nlm.nih.gov/pubmed/30405871
http://dx.doi.org/10.1155/2018/4582590
work_keys_str_mv AT zengyanan amethodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT lujunsheng amethodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT changxinyu amethodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT liuyuan amethodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT huxiaodong amethodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT sukangyan amethodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT chenxiayu amethodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT zengyanan methodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT lujunsheng methodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT changxinyu methodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT liuyuan methodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT huxiaodong methodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT sukangyan methodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy
AT chenxiayu methodtoimprovetheimagingqualityindualwavelengthdigitalholographicmicroscopy