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Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia

Extreme and unusual ecosystems such as isolated ancient caves are considered as potential tools for the discovery of novel natural products with biological activities. Actinobacteria that inhabit these unusual ecosystems are examined as a promising source for the development of new drugs. In this st...

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Autores principales: Voytsekhovskaya, Irina V., Axenov-Gribanov, Denis V., Murzina, Svetlana A., Pekkoeva, Svetlana N., Protasov, Eugeniy S., Gamaiunov, Stanislav V., Timofeyev, Maxim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204239/
https://www.ncbi.nlm.nih.gov/pubmed/30386707
http://dx.doi.org/10.7717/peerj.5832
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author Voytsekhovskaya, Irina V.
Axenov-Gribanov, Denis V.
Murzina, Svetlana A.
Pekkoeva, Svetlana N.
Protasov, Eugeniy S.
Gamaiunov, Stanislav V.
Timofeyev, Maxim A.
author_facet Voytsekhovskaya, Irina V.
Axenov-Gribanov, Denis V.
Murzina, Svetlana A.
Pekkoeva, Svetlana N.
Protasov, Eugeniy S.
Gamaiunov, Stanislav V.
Timofeyev, Maxim A.
author_sort Voytsekhovskaya, Irina V.
collection PubMed
description Extreme and unusual ecosystems such as isolated ancient caves are considered as potential tools for the discovery of novel natural products with biological activities. Actinobacteria that inhabit these unusual ecosystems are examined as a promising source for the development of new drugs. In this study we focused on the preliminary estimation of fatty acid composition and antibacterial properties of culturable actinobacteria isolated from water surface of underground lakes located in Badzheyskaya and Okhotnichya caves in Siberia. Here we present isolation of 17 strains of actinobacteria that belong to the Streptomyces, Nocardia and Nocardiopsis genera. Using assays for antibacterial and antifungal activities, we found that a number of strains belonging to the genus Streptomyces isolated from Badzheyskaya cave demonstrated inhibition activity against bacteria and fungi. It was shown that representatives of the genera Nocardia and Nocardiopsis isolated from Okhotnichya cave did not demonstrate any tested antibiotic properties. However, despite the lack of antimicrobial and fungicidal activity of Nocardia extracts, those strains are specific in terms of their fatty acid spectrum. When assessing fatty acid profile, we found that polyunsaturated fatty acids were quantitatively dominant in extracts of Nocardia sp. and Streptomyces sp. grown in different media. Saturated fatty acids were the second most abundant type in the fatty acid profile. It was due to palmitic acid. Also, a few monounsaturated fatty acids were detected. The obtained materials can become a basis for development of approaches to use bacteria isolated from caves as a biological sources of bioactive compounds to create medical and veterinary drugs.
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spelling pubmed-62042392018-10-31 Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia Voytsekhovskaya, Irina V. Axenov-Gribanov, Denis V. Murzina, Svetlana A. Pekkoeva, Svetlana N. Protasov, Eugeniy S. Gamaiunov, Stanislav V. Timofeyev, Maxim A. PeerJ Agricultural Science Extreme and unusual ecosystems such as isolated ancient caves are considered as potential tools for the discovery of novel natural products with biological activities. Actinobacteria that inhabit these unusual ecosystems are examined as a promising source for the development of new drugs. In this study we focused on the preliminary estimation of fatty acid composition and antibacterial properties of culturable actinobacteria isolated from water surface of underground lakes located in Badzheyskaya and Okhotnichya caves in Siberia. Here we present isolation of 17 strains of actinobacteria that belong to the Streptomyces, Nocardia and Nocardiopsis genera. Using assays for antibacterial and antifungal activities, we found that a number of strains belonging to the genus Streptomyces isolated from Badzheyskaya cave demonstrated inhibition activity against bacteria and fungi. It was shown that representatives of the genera Nocardia and Nocardiopsis isolated from Okhotnichya cave did not demonstrate any tested antibiotic properties. However, despite the lack of antimicrobial and fungicidal activity of Nocardia extracts, those strains are specific in terms of their fatty acid spectrum. When assessing fatty acid profile, we found that polyunsaturated fatty acids were quantitatively dominant in extracts of Nocardia sp. and Streptomyces sp. grown in different media. Saturated fatty acids were the second most abundant type in the fatty acid profile. It was due to palmitic acid. Also, a few monounsaturated fatty acids were detected. The obtained materials can become a basis for development of approaches to use bacteria isolated from caves as a biological sources of bioactive compounds to create medical and veterinary drugs. PeerJ Inc. 2018-10-25 /pmc/articles/PMC6204239/ /pubmed/30386707 http://dx.doi.org/10.7717/peerj.5832 Text en ©2018 Voytsekhovskaya et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Voytsekhovskaya, Irina V.
Axenov-Gribanov, Denis V.
Murzina, Svetlana A.
Pekkoeva, Svetlana N.
Protasov, Eugeniy S.
Gamaiunov, Stanislav V.
Timofeyev, Maxim A.
Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia
title Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia
title_full Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia
title_fullStr Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia
title_full_unstemmed Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia
title_short Estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from Badzheyskaya and Okhotnichya caves in Siberia
title_sort estimation of antimicrobial activities and fatty acid composition of actinobacteria isolated from water surface of underground lakes from badzheyskaya and okhotnichya caves in siberia
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204239/
https://www.ncbi.nlm.nih.gov/pubmed/30386707
http://dx.doi.org/10.7717/peerj.5832
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