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Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas

Vegetable oil is an important renewable resource for dietary consumption for human and livestock, and more recently for biodiesel production. Lipid traits in crops are controlled by multiple quantitative trait loci (QTLs) and each of them has a small effect on lipid traits. So far, there is limited...

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Autores principales: Ye, Jian, Wang, Chunming, Sun, Yanwei, Qu, Jing, Mao, Huizhu, Chua, Nam-Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204399/
https://www.ncbi.nlm.nih.gov/pubmed/30405653
http://dx.doi.org/10.3389/fpls.2018.01479
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author Ye, Jian
Wang, Chunming
Sun, Yanwei
Qu, Jing
Mao, Huizhu
Chua, Nam-Hai
author_facet Ye, Jian
Wang, Chunming
Sun, Yanwei
Qu, Jing
Mao, Huizhu
Chua, Nam-Hai
author_sort Ye, Jian
collection PubMed
description Vegetable oil is an important renewable resource for dietary consumption for human and livestock, and more recently for biodiesel production. Lipid traits in crops are controlled by multiple quantitative trait loci (QTLs) and each of them has a small effect on lipid traits. So far, there is limited success to increase lipid yield and improve lipid quality in plants. Here, we reported the identification of a homolog of APETALA2 (AP2) transcription factor WRINKLED1 (JcWRI1) from an oleaginous plant Jatropha curcas and characterized its function in Jatropha and Arabidopsis thaliana. Using physical mapping data, we located JcWRI1 in a QTL region specifying high oleate and lipid content in Jatropha. Overexpression of JcWRI1 in Jatropha elevated seed lipid content and increased seed mass. Lipid profile in seeds of over-expression plants showed higher oleate content which will be beneficial to improve biodiesel quality. Overexpression of JcWRI1 activated lipid-related gene expression and JcWRI1 was shown to directly bind to the AW-box of promoters of some of these genes. In conclusion, we were able to increase seed lipid content and improve seed lipid quality in Jatropha by manipulating one key transcription factor JcWRI1.
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spelling pubmed-62043992018-11-07 Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas Ye, Jian Wang, Chunming Sun, Yanwei Qu, Jing Mao, Huizhu Chua, Nam-Hai Front Plant Sci Plant Science Vegetable oil is an important renewable resource for dietary consumption for human and livestock, and more recently for biodiesel production. Lipid traits in crops are controlled by multiple quantitative trait loci (QTLs) and each of them has a small effect on lipid traits. So far, there is limited success to increase lipid yield and improve lipid quality in plants. Here, we reported the identification of a homolog of APETALA2 (AP2) transcription factor WRINKLED1 (JcWRI1) from an oleaginous plant Jatropha curcas and characterized its function in Jatropha and Arabidopsis thaliana. Using physical mapping data, we located JcWRI1 in a QTL region specifying high oleate and lipid content in Jatropha. Overexpression of JcWRI1 in Jatropha elevated seed lipid content and increased seed mass. Lipid profile in seeds of over-expression plants showed higher oleate content which will be beneficial to improve biodiesel quality. Overexpression of JcWRI1 activated lipid-related gene expression and JcWRI1 was shown to directly bind to the AW-box of promoters of some of these genes. In conclusion, we were able to increase seed lipid content and improve seed lipid quality in Jatropha by manipulating one key transcription factor JcWRI1. Frontiers Media S.A. 2018-10-22 /pmc/articles/PMC6204399/ /pubmed/30405653 http://dx.doi.org/10.3389/fpls.2018.01479 Text en Copyright © 2018 Ye, Wang, Sun, Qu, Mao and Chua. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ye, Jian
Wang, Chunming
Sun, Yanwei
Qu, Jing
Mao, Huizhu
Chua, Nam-Hai
Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas
title Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas
title_full Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas
title_fullStr Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas
title_full_unstemmed Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas
title_short Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas
title_sort overexpression of a transcription factor increases lipid content in a woody perennial jatropha curcas
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204399/
https://www.ncbi.nlm.nih.gov/pubmed/30405653
http://dx.doi.org/10.3389/fpls.2018.01479
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